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Sensitive Electrochemical Detection of the Hydroxyl Radical Using Enzyme-catalyzed Redox Cycling

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Abstract

Enzyme-catalyzed signal amplification was introduced to the electrochemical detection of the OH radical. In the presence of phenol as a trapping agent, glucose as a substrate, and pyrroloquinoline quinone-containing glucose dehydrogenase (PQQ-GDH) as a catalyst, the current signal for the trapping adducts (catechol and hydroquinone) produced by the hydroxylation of phenol could be amplified and detected sensitively. The limit of detection (S/N = 3) for catechol was 8 nM. The trapping efficiency of phenol was also estimated.

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Authors and Affiliations

  1. International Young Researchers Empowerment Center, Shinshu University, Matsumoto, 390-8621, Nagano, Japan

    Hirosuke Tatsumi

  2. Department of Chemistry, Faculty of Science, Shinshu University, Matsumoto, 390-8621, Nagano, Japan

    Naoya Osaku

Authors
  1. Hirosuke Tatsumi
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  2. Naoya Osaku
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Correspondence to Hirosuke Tatsumi.

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Tatsumi, H., Osaku, N. Sensitive Electrochemical Detection of the Hydroxyl Radical Using Enzyme-catalyzed Redox Cycling. ANAL. SCI. 27, 1065–1067 (2011). https://doi.org/10.2116/analsci.27.1065

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  • DOI: https://doi.org/10.2116/analsci.27.1065

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