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Time-dependent Changes in Concentration of Two Clinically used Acetylcholinesterase Reactivators (HI-6 and Obidoxime) in Rat Plasma Determined by HPLC Techniques after in vivo Administration

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Abstract

A simple and reliable HPLC method for determination of rat plasma levels of clinically used acetylcholinesterase (AChE) reactivators (HI-6 and obidoxime) is presented in our study. Separation was carried out by HPLC using an octadecyl silica stationary phase and a mobile phase consisting of 24% acetonitrile and containing 5 mM sodium octanesulfonate and 5 mM tetramethylammonium chloride (pH 2.3). Following intramuscular administration of equimolar doses of both oximes (22.23 mg/kg), the maximum of HI-6 concentration in rat plasma was reached in about 20 min giving 15.26 ± 1.71 µg/mL. The distribution of obidoxime was fast; the single maximum 23.62 ± 3.563 µg/mL was recorded at about 10 min. HPLC with UV detection presented in our study is a general method which could be applied for quick measurements of bisquaternary AChE reactivators in rat plasma.

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Authors and Affiliations

  1. Department of Toxicology, Faculty of Health Sciences, University of Defence, Trebesska 1575, 500 01, Hradec Kralove, Czech Republic

    Jana Zdarova Karasova

  2. Center of Advances Studies, Faculty of Health Sciences, University of Defence, Trebesska 1575, 500 01, Hradec Kralove, Czech Republic

    Ladislav Novotny & Kamil Kuca

  3. Department of Public Health, Faculty of Health Sciences, University of Defence, Trebesska 1575, 500 01, Hradec Kralove, Czech Republic

    Karel Antos

  4. Radioisotope Laboratories and Vivarium, Faculty of Medicine, Charles University, Simkova 870, 500 38, Hradec Kralove, Czech Republic

    Helena Zivna

  5. Department of Chemistry, Faculty of Sciences, J. E. Purkinje University, Horeni 13, 400 96, Usti nad Labem, Czech Republic

    Kamil Kuca

Authors
  1. Jana Zdarova Karasova
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  2. Ladislav Novotny
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  3. Karel Antos
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  4. Helena Zivna
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  5. Kamil Kuca
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Correspondence to Jana Zdarova Karasova.

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Zdarova Karasova, J., Novotny, L., Antos, K. et al. Time-dependent Changes in Concentration of Two Clinically used Acetylcholinesterase Reactivators (HI-6 and Obidoxime) in Rat Plasma Determined by HPLC Techniques after in vivo Administration. ANAL. SCI. 26, 63–67 (2010). https://doi.org/10.2116/analsci.26.63

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  • DOI: https://doi.org/10.2116/analsci.26.63

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