Skip to main content

Advertisement

SpringerLink
Log in

1H NMR-based Metabonomic Analysis of Metabolic Changes in Streptozotocin-induced Diabetic Rats

  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

Diabetes mellitus is a complex metabolic disorder characterized by chronic hyperglycemia, hypoinsulinemia, and ketosis. To access the biochemical process of diabetes, we applied quantitative 1H NMR-based metabonomics to analyze urine, serum, and liver extracts from streptozotocin-induced diabetic rats. Principle component analysis (PCA) of 1H NMR spectra disclosed metabolic pattern differences between diabetic and control rats, and identified the related metabolic changes. The PCA scores plot demonstrated that the diabetic group could be distinguished from the control group, indicating that the metabolic characteristics of the two groups were markedly different. Our work reveals the accumulation of triglycerides, fatty acids and acetoacetate in diabetic rats, and may provide an efficient, convenient way for evaluating the pathological state and biochemical process of diabetes mellitus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. M. van der Graaf, S. W. Janssen, J. J. van Asten, A. R. Hermus, C. G. Sweep, J. A. Pikkemaat, G. J. Martens, and A. Heerschap, NMR Biomed., 2004, 17, 405.

    Article  CAS  PubMed  Google Scholar 

  2. S. Wild, G. Roglic, A. Green, R. Sicree, and H. King, Diabetes Care, 2004, 27, 1047.

    Article  PubMed  Google Scholar 

  3. G. G. Pinkse, O. H. Tysma, C. A. Bergen, M. G. Kester, F. Ossendorp, P. A. van Veelen, B. Keymeulen, D. Pipeleers, J. W. Drijfhout, and B. O. Roep, Proc. Natl. Acad. Sci. U. S. A., 2005, 102, 18425.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. A. Junod, A. E. Lambert, W. Stauffacher, and A. E. Renold, J. Clin. Invest., 1969, 48, 2129.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. P. Chen and J. Liu, Adv. Ther., 2007, 24, 1036.

    Article  CAS  PubMed  Google Scholar 

  6. J. Gil, M. Miralpeix, J. Carreras, and R. Bartrons, J. Biol. Chem., 1988, 263, 1868.

    Article  CAS  PubMed  Google Scholar 

  7. A. Mohammad, V. Sharma, and J. H. McNeill, Mol. Cell Biochem., 2002, 233, 139.

    Article  CAS  PubMed  Google Scholar 

  8. W. K. Hou, Y. X. Xian, L. Zhang, H. Lai, X. G. Hou, Y. X. Xu, T. Yu, F. Y. Xu, J. Song, C. L. Fu, W. W. Zhang, and L. Chen, Chin. Med. J. (Engl.), 2007, 120, 1704.

    Article  CAS  PubMed  Google Scholar 

  9. N. H. Ugochukwu and N. E. Babady, Life Sci., 2003, 73, 1925.

    Article  CAS  PubMed  Google Scholar 

  10. F. Belfiore, S. Iannello, R. Campione, and G. Volpicelli, Diabetes Res., 1989, 10, 183.

    CAS  PubMed  Google Scholar 

  11. N. Attia, S. Caprio, T. W. Jones, R. Heptulla, J. Holcombe, D. Silver, R. S. Sherwin, and W. V. Tamborlane, J. Clin. Endocrinol. Metab., 1999, 84, 2324.

    CAS  PubMed  Google Scholar 

  12. K. S. Nair, D. Halliday, and J. S. Garrow, Diabetologia, 1984, 27, 13.

    Article  CAS  PubMed  Google Scholar 

  13. J. K. Nicholson, J. C. Lindon, and E. Holmes, Xenobiotica, 1999, 29, 1181.

    Article  CAS  PubMed  Google Scholar 

  14. N. G. Psihogios, I. F. Gazi, M. S. Elisaf, K. I. Seferiadis, and E. T. Bairaktari, NMR Biomed., 2008, 21, 195.

    Article  CAS  PubMed  Google Scholar 

  15. J. L. Griffin and A. Vidal-Puig, Physiol. Genomics, 2008, 34, 1.

    Article  CAS  PubMed  Google Scholar 

  16. J. Yang, G. Xu, Q. Hong, H. M. Liebich, K. Lutz, R. M. Schmulling, and H. G. Wahl, J. Chromatogr, B: Anal. Technol. Biomed. Life Sci., 2004, 813, 53.

    Article  CAS  Google Scholar 

  17. M. van Doorn, J. Vogels, A. Tas, E. J. van Hoogdalem, J. Burggraaf, A. Cohen, and J. van der Greef, Br. J. Clin. Pharmacol., 2007, 63, 562.

    Article  CAS  PubMed  Google Scholar 

  18. V. P. Mäkinen, P. Soininen, C. Forsblom, M. Parkkonen, P. Ingman, K. Kaski, P. H. Groop, and M. Ala-Korpela, Magma, 2006, 19, 281.

    Article  PubMed  Google Scholar 

  19. V. P. Makinen, P. Soininen, C. Forsblom, M. Parkkonen, P. Ingman, K. Kaski, P. H. Groop, and M. Ala-Korpela, Mol. Syst. Biol., 2008, 4, 167.

    Article  PubMed  PubMed Central  Google Scholar 

  20. S. Zhang, G. A. Nagana Gowda, V. Asiago, N. Shanaiah, C. Barbas, and D. Raftery, Anal. Biochem., 2008, 383, 76.

    Article  CAS  PubMed  Google Scholar 

  21. H. Gao, B. Dong, X. Liu, H. Xuan, Y. Huang, and D. Lin, Anal. Chim. Acta, 2008, 624, 269.

    Article  CAS  PubMed  Google Scholar 

  22. O. Beckonert, H. C. Keun, T. M. Ebbels, J. Bundy, E. Holmes, J. C. Lindon, and J. K. Nicholson, Nat. Protocol., 2007, 2, 2692.

    Article  CAS  Google Scholar 

  23. J. K. Nicholson, P. J. Foxall, M. Spraul, R. D. Farrant, and J. C. Lindon, Anal. Chem., 1995, 67, 793.

    Article  CAS  PubMed  Google Scholar 

  24. B. M. Beckwith-Hall, J. K. Nicholson, A. W. Nicholls, P. J. Foxall, J. C. Lindon, S. C. Connor, M. Abdi, J. Connelly, and E. Holmes, Chem. Res. Toxicol., 1998, 11, 260.

    Article  CAS  PubMed  Google Scholar 

  25. E. Suthagar, S. Soudamani, S. Yuvaraj, A. Ismail Khan, M. M. Aruldhas, and K. Balasubramanian, Biomed. Pharmacother., 2008, 63, 43.

    Article  PubMed  Google Scholar 

  26. K. M. Korenblat, E. Fabbrini, B. S. Mohammed, and S. Klein, Gastroenterology, 2008, 134, 1369.

    Article  CAS  PubMed  Google Scholar 

  27. M. E. Brosnan, B. V. Roebothan, and D. E. Hall, Biochem. J., 1980, 190, 395.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. T. Szkudelski, Physiol. Res., 2001, 50, 537.

    CAS  PubMed  Google Scholar 

  29. M. Elsner, B. Guldbakke, M. Tiedge, R. Munday, and S. Lenzen, Diabetologia, 2000, 43, 1528.

    Article  CAS  PubMed  Google Scholar 

  30. E. M. de Cavanagh, L. Ferder, J. E. Toblli, B. Piotrkowski, I. Stella, C. G. Fraga, and F. Inserra, Am. J. Physiol. Heart Circ. Physiol., 2008, 294, H456.

    Article  CAS  PubMed  Google Scholar 

  31. D. An and B. Rodrigues, Am. J. Physiol. Heart Circ. Physiol., 2006, 291, H1489.

    Article  CAS  PubMed  Google Scholar 

  32. L. Fritsche, C. Weigert, H. U. Haring, and R. Lehmann, Curr. Med. Chem., 2008, 15, 1316.

    Article  CAS  PubMed  Google Scholar 

  33. J. T. Brosnan, K. C. Man, D. E. Hall, S. A. Colbourne, and M. E. Brosnan, Am. J. Physiol., 1983, 244, E151.

    CAS  PubMed  Google Scholar 

  34. M. R. Taskinen, Diabetes Metab. Rev., 1987, 3, 551.

    Article  CAS  PubMed  Google Scholar 

  35. G. Shepherd, M. C. Cam, N. Sambandam, M. A. Abrahani, and B. Rodrigues, Hypertension, 1998, 31, 878.

    Article  CAS  PubMed  Google Scholar 

  36. M. Gotoh, C. Li, M. Yatoh, N. Okabayashi, S. Habu, and Y Hirooka, Brain Res., 2006, 1073 - 1074, 497.

    Article  PubMed  Google Scholar 

  37. V. Sharma, P. Dhillon, R. Wambolt, H. Parsons, R. Brownsey, M. F. Allard, and J. H. McNeill, Am. J. Physiol. Heart Circ. Physiol., 2008, 294, H1609.

    Article  CAS  PubMed  Google Scholar 

  38. N. J. Waters, C. J. Waterfield, R. D. Farrant, E. Holmes, and J. K. Nicholson, Chem. Res. Toxicol., 2005, 18, 639.

    Article  CAS  PubMed  Google Scholar 

  39. V. Vats, S. P. Yadav, and J. K. Grover, J. Ethnopharmacol., 2003, 85, 237.

    Article  CAS  PubMed  Google Scholar 

  40. J. Shearer, G. Duggan, A. Weljie, D. S. Hittel, D. H. Wasserman, and H. J. Vogel, Diabetes Obes. Metab., 2008, 10, 950.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. K. P. Palmano, P. H. Whiting, and J. N. Hawthorne, Biochem. J., 1977, 167, 229.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  42. E. P. Wijekoon, C. Skinner, M. E. Brosnan, and J. T. Brosnan, Can. J. Physiol. Pharmacol., 2004, 82, 506.

    Article  CAS  PubMed  Google Scholar 

  43. H. J. Atherton, N. J. Bailey, W. Zhang, J. Taylor, H. Major, J. Shockcor, K. Clarke, and J. L. Griffin, Physiol. Genomics, 2006, 27, 178.

    Article  CAS  PubMed  Google Scholar 

  44. P. C. Nicholas, D. Kim, F. T. Crews, and J. M. Macdonald, Chem. Res. Toxicol., 2008, 21, 408.

    Article  CAS  PubMed  Google Scholar 

  45. F. Belfiore, A. M. Rabuazzo, S. Iannello, R. Campione, and D. Vasta, Biochem. Med. Metab. Biol., 1986, 35, 149.

    Article  CAS  PubMed  Google Scholar 

  46. C. L. Gavaghan, E. Holmes, E. Lenz, I. D. Wilson, and J. K. Nicholson, FEBS Lett., 2000, 484, 169.

    Article  CAS  PubMed  Google Scholar 

  47. I. Messana, F. Forni, F. Ferrari, C. Rossi, B. Giardina, and C. Zuppi, Clin. Chem., 1998, 44, 1529.

    Article  CAS  PubMed  Google Scholar 

  48. A. R. Saltiel and C. R. Kahn, Nature, 2001, 414, 799.

    Article  CAS  PubMed  Google Scholar 

  49. D. L. Bloxam, Br. J. Nutr., 1972, 27, 249.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Shanghai Institute of Materia Medica, The Chinese Academy of Sciences, Shanghai, 201203, P. R. China

    Liangcai Zhao, Xia Liu & Donghai Lin

  2. School of Pharmacy, Analysis and Testing Center, Wenzhou Medical College, Wenzhou, 325035, P. R. China

    Liyun Xie & Hongchang Gao

  3. The Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, P. R. China

    Donghai Lin

Authors
  1. Liangcai Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xia Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Liyun Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hongchang Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Donghai Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Hongchang Gao or Donghai Lin.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhao, L., Liu, X., Xie, L. et al. 1H NMR-based Metabonomic Analysis of Metabolic Changes in Streptozotocin-induced Diabetic Rats. ANAL. SCI. 26, 1277–1282 (2010). https://doi.org/10.2116/analsci.26.1277

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.26.1277

Search

Navigation