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High-Performance Liquid-Chromatographic Separation of Ofloxacin Using a Chiral Stationary Phase

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Abstract

A new chiral liquid-chromatographic method was developed for the enantiomeric separation of ofloxacin with a fluorescence detector. The enantiomers of ofloxacin were baseline resolved on a Chiralcel OD-H (250 mm × 4.6 mm, 5 μm) column using a mobile-phase system containing hexane–ethanol–methanol–acetic acid–diethylamine (70/20/10/0.45/0.05, v/v/v/v/v). The presence of diethylamine in the mobile phase has played an important role in enhancing the chromatographic efficiency and resolution between enantiomers.

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Authors and Affiliations

  1. Central Laboratory, Beijing Center for Disease Prevention & Control, Beijing, 100013, China

    Xiaojie Sun, Bing Shao & Jing Zhang

  2. College of Life Sciences, Xiamen University, Xiamen, Fujian, 361005, China

    Di Wu

Authors
  1. Xiaojie Sun
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  2. Di Wu
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  3. Bing Shao
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  4. Jing Zhang
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Corresponding author

Correspondence to Bing Shao.

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Sun, X., Wu, D., Shao, B. et al. High-Performance Liquid-Chromatographic Separation of Ofloxacin Using a Chiral Stationary Phase. ANAL. SCI. 25, 931–933 (2009). https://doi.org/10.2116/analsci.25.931

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  • DOI: https://doi.org/10.2116/analsci.25.931

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