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Selection of the Optimal Solvent Grade for the Mobile Phase in HPLC with Electrochemical Detection Based on FIMI Theory

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Abstract

The optimum conditions of the mobile phase for HPLC with electrochemical detection (HPLC-ECD) were selected from among solvents of different grades with the standard deviation (SD) of area measurements based on FUMI (function of mutual information) theory as a criterion. In HPLC-ECD for determining baicalin and baicalein using a phosphoric acid–methanol–water mixture (0.5:60:40, v/v/v) as a mobile phase, the effects of three grades of phosphoric acid, of five grades of methanol, and of seven grades of water on the measurement precision were examined. The mixture, if characterized by the minimum SD of measurements, was selected as the optimal mobile phase. This selection method saves considerable amounts of chemicals and experimental time, and would be a useful exploration technique for the routine check and troubleshooting of HPLC-ECD.

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Authors and Affiliations

  1. School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan

    Akira Kotani, Yanan Yuan & Fumiyo Kusu

  2. Institute of Chinese Material Medica, China Academy of Chinese Medical Sciences, 16 Dongzhimennei Nanxiaojie, Beijing, 100700, China

    Yanan Yuan & Bin Yang

  3. National Institute of Health Sciences, 1-18-1 Kami-Yoga, Setagaya, Tokyo, 158-8501, Japan

    Yuzuru Hayashi & Rieko Matsuda

Authors
  1. Akira Kotani
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  2. Yanan Yuan
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  3. Bin Yang
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  4. Yuzuru Hayashi
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  5. Rieko Matsuda
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  6. Fumiyo Kusu
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Correspondence to Akira Kotani.

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Kotani, A., Yuan, Y., Yang, B. et al. Selection of the Optimal Solvent Grade for the Mobile Phase in HPLC with Electrochemical Detection Based on FIMI Theory. ANAL. SCI. 25, 925–929 (2009). https://doi.org/10.2116/analsci.25.925

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  • DOI: https://doi.org/10.2116/analsci.25.925

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