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Highly Selective and Simple Method for Determination of Polythiols Based on Liquid Chromatography with Postcolumn Excimer Fluorescence Derivatization

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Abstract

An LC postcolumn derivatization method for determination of polythiols has been developed. This method involves separation using reversed-phase LC, postcolumn derivatization with N-(1-pyrenyl)maleimide, and excimer fluorescence detection. Analytes with a polythiol structure were converted into corresponding polypyrene-labeled derivatives, and the derivatives exhibited intramolecular excimer fluorescence (440 – 520 nm). In this study, dimercaprol and dithiothreitol were used as model polythiols. This polythiol analysis method is simple; it is also highly selective and sensitive and yields good calibration curves.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Johnan, Fukuoka, 814-0180, Japan

    Hideyuki Yoshida, Maki Sudo, Kenichiro Todoroki, Hitoshi Nohta & Masatoshi Yamaguchi

  2. Physical Activity Institute, Fukuoka University, 8-19-1 Nanakuma, Johnan, Fukuoka, 814-0180, Japan

    Hideyuki Yoshida & Masatoshi Yamaguchi

Authors
  1. Hideyuki Yoshida
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  2. Maki Sudo
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  3. Kenichiro Todoroki
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  4. Hitoshi Nohta
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  5. Masatoshi Yamaguchi
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Corresponding author

Correspondence to Masatoshi Yamaguchi.

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Yoshida, H., Sudo, M., Todoroki, K. et al. Highly Selective and Simple Method for Determination of Polythiols Based on Liquid Chromatography with Postcolumn Excimer Fluorescence Derivatization. ANAL. SCI. 25, 829–832 (2009). https://doi.org/10.2116/analsci.25.829

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  • DOI: https://doi.org/10.2116/analsci.25.829

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