Skip to main content
SpringerLink
Log in

Electrochemical Study of Functionalization on the Surface of a Chitin/Platinum-modified Glassy Carbon Paste Electrode

  • Notes
  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

To functionalize chitin surfaces using proteins, we developed a glucose oxidase (GOD)-chitin/platinum-modified glassy carbon paste electrode (GCPE) as a model. In a weakly acidic solution, negatively charged GOD were immobilized by the protonated acetylamide groups on chitin. When the electrode was immersed in a solution containing GOD, the enzyme was readily immobilized due to the electrostaic interaction. In addition, measurements were performed using electrodes made with powders of different sizes because sensor performance depends on the particle sizes of glassy carbon powder.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Y. Wang, Y. Ke, L. Ren, G. Wu, X. Chen, and Q. Zhao, J. Biomed. Mater. Res., Part A, 2009, 88A, 616.

  2. J. Skopinska-Wisniewska, A. Sionkowska, A. Kaminska, A. Kaznica, R. Jachimiak, and T. Drewa, Appl. Surf. Sci., 2009, 255, 8286.

    Article  CAS  Google Scholar 

  3. Y. Stark, S. Bruns, F. Stahl, C. Kasper, M. Wesemann, C. Grothe, and T. Scheper, J. Biomed. Mater. Res., Part A, 2007, 85A, 1.

  4. B. Krajewska, Enzyme Microb. Technol., 2004, 35, 126.

    Article  CAS  Google Scholar 

  5. S. C. Fernandes, I. R. W. Z. Oliveira, and I. C. Vieira, Enzyme Microb. Technol., 2007, 40, 661.

    Article  CAS  Google Scholar 

  6. J. Cruz, M. Kawasaki, and W. Gorski, Anal. Chem., 2000, 72, 680.

    Article  CAS  Google Scholar 

  7. C. Zhao, Y. Meng, C. Shao, L. Wan, and K. Jiao, Electroanalysis, 2008, 20, 520.

    Article  CAS  Google Scholar 

  8. Y. Liu, M. Wang, F. Zhao, Z. Xu, and S. Dong, Biosens. Bioelectron., 2006, 21, 984.

    Article  CAS  Google Scholar 

  9. Y. Liu, X. Qu, H. Guo, H. Chen, B. Liu, and S. Dong, Biosens. Bioelectron., 2006, 21, 2195.

    Article  CAS  Google Scholar 

  10. C. Hao, L. Ding, X. Zhang, and H. Ju, Anal. Chem., 2007, 79, 4442.

    Article  CAS  Google Scholar 

  11. X. Yang, L. Hua, H. Gong, and S. N. Tan, Anal. Chim. Acta, 2003, 478, 67.

    Article  CAS  Google Scholar 

  12. J. Wang, Ü. A. Kirgöz, J.-W. Mo, J. Lu, A. N. Kawde, and A. Muck, Electrochem. Commun., 2003, 3, 203.

    Article  Google Scholar 

  13. T. Ikeda, H. Hamada, K. Mili, and M. Senda, Agr. Biol. Chem., 1985, 49, 541.

    CAS  Google Scholar 

  14. J. Wang, L. H. Wu, Z. L. Lu, R. L. Li, and J. Sanchez, Anal. Chim. Acta, 1990, 228, 251.

    Article  CAS  Google Scholar 

  15. C. I. Ladiu, J. R. Garcia, I. C. Popescu, and L. Gorton, Rev. Roum. Chim., 2007, 52, 67.

    CAS  Google Scholar 

  16. K. Sugawara, T. Takano, H. Fukushi, S. Hoshi, K. Akatsuka, H. Kuramitz, and S. Tanaka, J. Electroanal. Chem., 2000, 482, 81.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Engineering, Maebashi Institute of Technology, 371-0816, Maebashi, Gunma, Japan

    Kazuharu Sugawara

  2. Faculty of Education, Gunma University, 371-8510, Maebashi, Gunma, Japan

    Asako Yugami

  3. Department of Chemical Engineering, Ichinoseki National College of Technology, 021-8511, Ichinoseki, Iwate, Japan

    Norifumi Terui

  4. Department of Environmental Biology and Chemistry, Faculty of Science, University of Toyama, 930-8555, Toyama, Japan

    Hideki Kuramitz

Authors
  1. Kazuharu Sugawara
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Asako Yugami
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Norifumi Terui
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hideki Kuramitz
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kazuharu Sugawara.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sugawara, K., Yugami, A., Terui, N. et al. Electrochemical Study of Functionalization on the Surface of a Chitin/Platinum-modified Glassy Carbon Paste Electrode. ANAL. SCI. 25, 1365–1368 (2009). https://doi.org/10.2116/analsci.25.1365

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.25.1365

Search

Navigation