Skip to main content
SpringerLink
Log in

Application of 4-Iodophenol-enhanced Luminol Chemiluminescence to Direct Detection of Horseradish Peroxidase Encapsulated in Liposomes

  • Notes
  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

4-Iodophenol was applied to an enhancer in the direct detection of horseradish peroxidase (HRP) encapsulated in liposomes by using luminol chemiluminescence (CL). Luminol, 4-iodophenol and hydrogen peroxide permeate into the inner phase of liposomes containing HRP, resulting in the progress of 4-iodophenol-enhanced luminol CL catalyzed by HRP in liposomes. The CL intensity observed in liposomes was a factor of 150 greater than that observed in a lipid-free bulk solution. The detection limit in the direct detection of HRP encapsulated in liposomes was sensitive by a factor of 30 compared with that in a lipid-free bulk solution. 4-Iodophenol effectively functioned as an enhancer in HRP-catalyzed luminol CL in liposomes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. V. P. Torchilin and V. Weissing, “Liposomes”, 2003, Oxford University Press, Oxford.

  2. R. J. Y. Ho, B. T. Rouse, and L. Haung, J. Biol. Chem., 1987, 262, 13979.

    Article  CAS  PubMed  Google Scholar 

  3. T. Kataoka, J. R. Williamson, and S. C. Kinsky, Biochim. Biophys. Acta, 1973, 298, 158.

    Article  CAS  PubMed  Google Scholar 

  4. H. Arakawa, M. Maeda, and A. Tsuji, Anal. Biochem., 1979, 97, 248.

    Article  CAS  PubMed  Google Scholar 

  5. T. Suita, T. Kamidate, M. Yonaiyama, and H. Watanabe, Anal. Sci., 1997, 13, 577.

    Article  CAS  Google Scholar 

  6. T. Kamidate, K. Komatsu, H. Tani, and A. Ishida, Anal. Sci., 2008, 24, 77.

    Article  Google Scholar 

  7. Y. Dotsikas and L. L. Yannis, Talanta, 2007, 71, 906.

    Article  CAS  PubMed  Google Scholar 

  8. G. H. G. Thorpe and L. J. Kricka, Methods Enzymol., 1986, 133, 331.

    Article  CAS  PubMed  Google Scholar 

  9. B. B. Kim, V. V. Pisarev, and A. M. Egorov, Anal. Biochem., 1991, 199, 1.

    Article  CAS  PubMed  Google Scholar 

  10. T. Kamidate, T. Suita, and H. Watanabe, Anal. Biochem., 1996, 241, 264.

    Article  CAS  PubMed  Google Scholar 

  11. A. M. Diaz, F. G. Sanchez, and J. A. G. Garcai, J. Biolumin. Chemilumin., 1998, 13, 75.

    Article  Google Scholar 

  12. P. M. Easton, A. C. Simmonds, A. Rakishev, A. H. Egorov, and L. P. Candeias, J. Am. Chem. Soc., 1996, 118, 6619.

    Article  CAS  Google Scholar 

  13. E. S. Wu, K. Jacobson, and D. Papahajdopoulos, Biochemistry, 1977, 16, 3936.

    Article  CAS  PubMed  Google Scholar 

  14. T. Kamidate, K. Komatsu, H. Tani, and A. Ishida, Luminescence, 2007, 22, 236.

    Article  CAS  PubMed  Google Scholar 

  15. M. C. Blok, L. L. Van, and J. D. E. Grier, Biochim. Biophys. Acta, 1977, 464, 509.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Biotechnology and Molecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, 060-8628, Japan

    Tamio Kamidate, Masumi Maruya, Hirofumi Tani & Akihiko Ishida

Authors
  1. Tamio Kamidate
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masumi Maruya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hirofumi Tani
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Akihiko Ishida
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kamidate, T., Maruya, M., Tani, H. et al. Application of 4-Iodophenol-enhanced Luminol Chemiluminescence to Direct Detection of Horseradish Peroxidase Encapsulated in Liposomes. ANAL. SCI. 25, 1163–1166 (2009). https://doi.org/10.2116/analsci.25.1163

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.25.1163

Search

Navigation