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Measurement of the Transport Activities of Bile Salt Export Pump Using LC-MS

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Abstract

The high performance liquid chromatography–electrospray ionization–mass spectrometry method has been applied to the measurement of bile acid transport activities in membrane vesicles obtained from a human bile salt export pump expressing Sf9 cells. The amounts of bile acids transported using the human bile salt export pump expressing Sf9 cells were determined using liquid chromatography–electrospray ionization–mass spectrometry method and the values of the kinetic parameters were determined to be comparable with those obtained using radioisotope-labeled substrates. The developed method was highly useful for the measurements of bile acid transport activities.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan

    Kana Yamaguchi, Tsuyoshi Murai & Takao Kurosawa

  2. GenoMembrane, Inc., 75-1 Ono, Tsurumi, Yokohama, 230-0046, Japan

    Hikaru Yabuuchi

Authors
  1. Kana Yamaguchi
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  2. Tsuyoshi Murai
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  3. Hikaru Yabuuchi
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  4. Takao Kurosawa
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Correspondence to Takao Kurosawa.

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Yamaguchi, K., Murai, T., Yabuuchi, H. et al. Measurement of the Transport Activities of Bile Salt Export Pump Using LC-MS. ANAL. SCI. 25, 1155–1158 (2009). https://doi.org/10.2116/analsci.25.1155

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  • DOI: https://doi.org/10.2116/analsci.25.1155

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