Skip to main content
SpringerLink
Log in

Practical Method for the Detection of Tetracyclines in Honey by HPLC and Derivative UV-Vis Spectra

  • Notes
  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

A technique to detect residual tetracyclines (TCs) in honey by HPLC and derivative UV-Vis spectra is examined in this study. Chromatograms of honey products often experience overlapped peaks and sloping background due to similar retention properties of components or to the presence of impurities. The derivatives provide resolution of overlapped peaks in an UV-Vis spectrum and eventually bring chromatograms that show pure elution behavior of TCs. Calibration models for oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) are built out of the derivative spectra of standard solutions of honey and their prediction abilities are examined. The results show that the calibration models for OTC, TC and CTC can yield sufficient levels for the determination. The technique reported here may be effectively used for quantitative determination of TCs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. W. H. H. Farrington, J. Tarbin, and G. Shearer, Food Addit. Contam., 1991, 8, 55.

    Article  CAS  PubMed  Google Scholar 

  2. J. Kochansky, Apidologyie, 2000, 31, 517.

    Article  CAS  Google Scholar 

  3. D. G. Tinkelman and S. A. Bock, Ann. Allergy, 1984, 53, 243.

    CAS  PubMed  Google Scholar 

  4. A. E. van den Bogaard and E. E. Stobberingh, Int. J. Antimicrob. Agents, 2000, 14, 327.

    Article  CAS  PubMed  Google Scholar 

  5. Z. Sáenz, M. Zarazaga, L. Brinas, M. Lantero, F. Ruiz-Larrea, and C. Torres, Int. J. Antimicrob. Agents, 2001, 18, 353.

    Article  PubMed  Google Scholar 

  6. U.S. Code of Federal Regulations, Title 21, Part 556, Sections 150, 500, and 720, 2003, Chap. 1, U.S. Government Printing Office,Washington D.C.

  7. The European Agency for the Evaluation of Medicinal Products, EMEA/MRL/023/95, http://www.emea.europa.eu/pdfs/vet/mrls/Tetracyclines(3)02395.pdf.

  8. Notification No. 499, 2005, Ministry of Health, Labour and Welfare, Tokyo, Japan.

  9. H. Oka, Y. Ikai, N. Kawamura, K. Uno, and M. Yamada, J. Chromatogr., 1987, 389, 417.

    Article  CAS  PubMed  Google Scholar 

  10. P. Viñas, N. Balsalobre, C. López-Erroz, and M. Hernándes-Córdoba, J. Chromatogr., A, 2004, 1022, 125.

    Article  PubMed  Google Scholar 

  11. T. Charoenraks, S. Chuanuwatanakul, K. Honda, Y. Yamaguchi, and O. Chailapakul, Anal. Sci., 2005, 21, 241.

    Article  CAS  PubMed  Google Scholar 

  12. H. Oka, Y. Ikai, N. Kawamura, K. Uno, M. Yamada, K. Harada, and M. Suzuki, J. Chromatogr., 1987, 400, 253.

    Article  CAS  PubMed  Google Scholar 

  13. W. Heering, E. Usleber, R. Dietrich, and E. Märtlbauer, Analyst [London], 1998, 123, 2759.

  14. K. Nakaya, Y. Kobayashi, and N. Tanahashi, J. Food Hyg. Soc. Jpn., 1991, 32, 43.

    Article  CAS  Google Scholar 

  15. A. Pena, N. Pelantova, C. M. Lino, M. I. N. Silveila, and P. Solich, J. Agric. Food Chem., 2005, 53, 3784.

    Article  CAS  PubMed  Google Scholar 

  16. G. Wan, H. Cui, H. Zheng, J. Zhou, L. Liu, and X. Yu, J. Chromatogr., B, 2005, 824, 57.

    Article  CAS  Google Scholar 

  17. H. Nakazawa, S. Ino, K. Kato, T. Watanabe, Y. Ito, and H. Oka, J. Chromatogr., B, 1999, 732, 55.

    Article  CAS  Google Scholar 

  18. A. Kaufmann, S. Roth, B. Ryser, M. Widmer, and D. Guggisberg, J. AOAC Int., 2002, 85, 853.

    Article  CAS  PubMed  Google Scholar 

  19. R. Ishii, M. Horie, M. Murayama, and T. Maitani, J. Food Hyg. Soc. Jpn., 2006, 47, 277.

    Article  CAS  Google Scholar 

  20. J. W. White, Jr., “Honey, The Hive and the Honeybee”, 1992, Dadant and Sons, USA.

  21. H. Shinzawa, S. Morita, Y. Ozaki, and R. Tsenkova, Appl. Spectrosc, 2006, 60, 884.

    Article  CAS  PubMed  Google Scholar 

  22. A. Yamamoto, A. Matsunaga, M. Ohto, E. Mizukami, K. Hayakawa, and M. Miyazaki, Analyst [London], 1995, 120, 377.

  23. A. Savitzky and M. J. E. Golay, Anal. Chem., 1964, 36, 1627.

    Article  CAS  Google Scholar 

  24. C. Bosch Ojeda and F. Sanchez Rojas, Anal. Chim. Acta, 2004, 518, 1.

    Article  CAS  Google Scholar 

  25. Director Notice Syoku-An No. 0124001, 2005, Ministry of Health, Labour and Welfare, Tokyo, Japan.

Download references

Author information

Authors and Affiliations

  1. Ibaraki Prefectural Institute of Public Health, Ibaraki, 310-0852, Japan

    Tadao Hakuta

  2. Research Institute of Instrumentation Frontier, Advanced Industrial Science and Technology (AIST), Aichi, 463-8560, Japan

    Hideyuki Shinzawa

  3. Department of Chemistry, School of Science and Technology, Kwansei-Gakuin University, Hyogo, 669-1337, Japan

    Yukihiro Ozaki

Authors
  1. Tadao Hakuta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hideyuki Shinzawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yukihiro Ozaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yukihiro Ozaki.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hakuta, T., Shinzawa, H. & Ozaki, Y. Practical Method for the Detection of Tetracyclines in Honey by HPLC and Derivative UV-Vis Spectra. ANAL. SCI. 25, 1149–1153 (2009). https://doi.org/10.2116/analsci.25.1149

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.25.1149

Search

Navigation