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A Tyrosinase Biosensor Based on ZnO Nanorod Clusters/ Nanocrystalline Diamond Electrodes for Biosensing of Phenolic Compounds

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Abstract

An amperometric biosensor was constructed by using ZnO nanorod clusters as platforms for immobilizing tyrosinase on the nanocrystalline diamond (NCD) electrodes. The results showed that ZnO nanorod clusters provided an advantageous microenvironment due to their favorable isoelectric point (IEP) for tyrosinase loading; immobilized tyrosinase generally retained its activity. The tyrosinase/ZnO/NCD electrode showed a linear response range of 1 – 210 and sensitivity of 179.9 µA mmol–1 cm–2 for p-cresol. The corresponding values were 1 – 190 and 90.2 for phenol, and 1 – 250 and 121.3 for 4-chlorophenol. The low detection limits were estimated to be 0.2 µM for p-cresol, 0.5 µM for phenol, and 0.4 µM for 4-chlorophenol (S/N = 3). The prepared enzyme electrode could keep 85% of its original activity after intermittent use for 4 weeks when stored in a dry state at 277 K. Therefore, the ZnO nanorod cluster thin films have potential applications as platforms to immobilize other enzymes and bioactive molecules in biosensors.

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Jishou University, Jishou, Hunan, 416000, P. R. China

    Jianwen Zhao & Wenbin Yan

  2. Laboratory of Organic Optoelectronic Functional Materials and Molecular Engineering, Technical Institute of Physics and Chemistry & Graduate University of Chinese Academy of Sciences, No. 2, Beiyitiao, Zhong-guan-cun, Haidian, Beijing, 100080, P. R. China

    Jinfang Zhi

  3. Deparment of Chemistry, Shangqiu Normal University, Shangqiu, Henan, 476000, P. R. China

    Yanli Zhou

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  1. Jianwen Zhao
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  2. Jinfang Zhi
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  3. Yanli Zhou
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  4. Wenbin Yan
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Correspondence to Jianwen Zhao.

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Zhao, J., Zhi, J., Zhou, Y. et al. A Tyrosinase Biosensor Based on ZnO Nanorod Clusters/ Nanocrystalline Diamond Electrodes for Biosensing of Phenolic Compounds. ANAL. SCI. 25, 1083–1088 (2009). https://doi.org/10.2116/analsci.25.1083

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  • DOI: https://doi.org/10.2116/analsci.25.1083

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