Abstract
Direct and quick molecular analysis of live plant single cells viewed under a video-microscope has been developed in their intact undamaged environment using nano-electrospray tip and mass spectrometers. This method has been successful in identifying specific molecules in plant live single-cell analysis by mass spectrometry, and opens the possibility to compare different cell types from different tissues with morphological evidence in undamaged plants.
References
T. Masujima, Anal. Chim. Acta, 1999, 33, 400.
M. Malone, R. A. Leigh, and A. D. Tomos, Plant Cell Environ., 1989, 12, 919.
A. Amantonico, J. Y. Oh, J. Sobek, M. Heinemann, and R. Zenobi, Angew. Chem., Int. Ed., 2008, 47, 5382.
H. Mizuno, N. Tsuyama, E. Tokunaga, and T. Masujima, Anal. Sci., 2008, 24, 559.
H. Mizuno, N. Tsuyama, T. Harada, and T. Masujima, J. Mass Spectrom., 2008, 43, 1692.
A. Makaro, E. Denisov, A. Kholomeev, W. Balschun, O. Lange, K. Strupat, and S. Horning, Anal. Chem., 2006, 78, 2113.
A. Makarov, E. Denisov, O. Lange, and S. Horning, J. Am. Soc. Mass Spectrom., 2006, 17, 977.
A. Gauvin, H. Lecomte, and J. Smadja, Flavour Frag. J., 2004, 19, 455.
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Lorenzo Tejedor, M., Mizuno, H., Tsuyama, N. et al. Direct Single-Cell Molecular Analysis of Plant Tissues by Video Mass Spectrometry. ANAL. SCI. 25, 1053–1055 (2009). https://doi.org/10.2116/analsci.25.1053
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DOI: https://doi.org/10.2116/analsci.25.1053