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Dual-Gradient Capillary Electrochromatography by Varying the Mobile Phase Composition and Applied Voltage

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Abstract

Dual-gradient capillary electrochromatography (DG-CEC) was developed to provide superior performance with regard to the separation of ionized analytes; in this method, both the eluent composition and the applied voltage are varied during the separation procedure. As for the gradient in the eluent composition, a shift in the pH is employed to control not only the electrophoretic mobility, but also the retention factor of the analytes. The dual-gradient method was shown to be effective in increasing the resolution and reducing the chromatographic period of ionized analytes. Fourteen kinds of o-phthalaldehyde labeled amino acids were separated within 8 min using DG-CEC with multistage enlargement in the applied voltage. The separation efficiency increased particularly for highly retained amino acids in the dual-gradient, as compared to those in the ordinary single-gradient for the eluent.

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Authors and Affiliations

  1. Department of Materials Science and Engineering, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso, Showa, Nagoya, 466-8555, Japan

    Tomoharu Sakai, Hiroyuki Nakagawa, Shinya Kitagawa & Hajime Ohtani

Authors
  1. Tomoharu Sakai
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  2. Hiroyuki Nakagawa
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  3. Shinya Kitagawa
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  4. Hajime Ohtani
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Corresponding author

Correspondence to Shinya Kitagawa.

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Sakai, T., Nakagawa, H., Kitagawa, S. et al. Dual-Gradient Capillary Electrochromatography by Varying the Mobile Phase Composition and Applied Voltage. ANAL. SCI. 24, 735–738 (2008). https://doi.org/10.2116/analsci.24.735

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  • DOI: https://doi.org/10.2116/analsci.24.735

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