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Microscopic Measurement of Circular Dichroism Spectra

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Abstract

A microscope device to measure the circular dichroism (CD) spectra of a specified microscopic region of chiral samples was constructed by combining of a couple of objective lenses and a CCD camera, which was installed in a sample chamber of a commercially available CD spectropolarimeter. By using this apparatus, high quality micro-CD spectra in the 60 × 60 μm region of samples could be measured. Micro-CD spectra of thin film of chiral DNA samples on glass and a natural kidney bean leaf were measured, and the potential of the micro-CD apparatus was successfully demonstrated.

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Authors and Affiliations

  1. Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043, Japan

    Aira Matsugaki, Hideaki Takechi, Hideaki Monjushiro & Hitoshi Watarai

Authors
  1. Aira Matsugaki
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  2. Hideaki Takechi
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  3. Hideaki Monjushiro
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  4. Hitoshi Watarai
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Corresponding author

Correspondence to Hitoshi Watarai.

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Matsugaki, A., Takechi, H., Monjushiro, H. et al. Microscopic Measurement of Circular Dichroism Spectra. ANAL. SCI. 24, 297–300 (2008). https://doi.org/10.2116/analsci.24.297

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  • DOI: https://doi.org/10.2116/analsci.24.297

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