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Redox Protein-Polymer Films for Simultaneous Determination of Ascorbic Acid and Hydrogen Peroxide

Analytical Sciences volume 24, pages 1105–1110 (2008)Cite this article

Abstract

Layer by layer films of protein and redox polymer were constructed and used to simultaneously analyze ascorbic acid and hydrogen peroxide. The films were made using hemoglobin and poly[4-vinylpyridine Os(bipyridine)2Cl]-co-ethylamine (Pos-Ea). The film growth was monitored using cyclic voltammetry, quartz crystal microbalance (QCM) and atomic force microscopy (AFM). Reversible pairs of oxidation-reduction peaks were observed using cyclic voltammetry corresponding to the OsII/OsIII from redox polymer and HbFeIII/HbFeII redox couples at 0.35 and –0.25 V vs. Ag/AgCl, respectively. The two redox centers were independent of each other. This enabled the simultaneous and independent determination of ascorbic acid and hydrogen. Peak currents were linearly related to concentration for both analytes in a mixture. The linear range of ascorbic acid was 0 – 1 mM (R2 = 0.9996, n = 5) at scan rate of 50 mV s−1 (sensitivity 3.5 mA/mM) while hydrogen peroxide linear range was 1.0 – 10.0 mM (R2 = 0.991, n = 6) with sensitivity of 1.85 mA/mM.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Rowan University, 201 Mullica Hill Road, 08028, Glassboro, NJ, USA

    Amos Mugweru, Hoang Khoa & Emmanuel Yawson

  2. Department of Chemistry, Missouri State University, 901 South National Ave, 65897, Springfield, MO, USA

    Sagar Tolani & Adam Wanekaya

Authors
  1. Amos Mugweru
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  2. Hoang Khoa
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  3. Emmanuel Yawson
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  4. Sagar Tolani
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  5. Adam Wanekaya
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Correspondence to Amos Mugweru.

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Mugweru, A., Khoa, H., Yawson, E. et al. Redox Protein-Polymer Films for Simultaneous Determination of Ascorbic Acid and Hydrogen Peroxide. ANAL. SCI. 24, 1105–1110 (2008). https://doi.org/10.2116/analsci.24.1105

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  • DOI: https://doi.org/10.2116/analsci.24.1105