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Development of a Surface-Reaction System in a Nanoliter Droplet Made by an Ink-jet Microchip

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Abstract

A surface-reaction system in a nanoliter water pool using an ink-jet microchip was developed. The reaction system in the nanodroplets formed on a poly(dimethylsiloxane) (PDMS) coated glass slide increased the diffusion-controlled reaction without using a nano-pump, specialized connector or highly sensitive detector. When nanoliter droplets were placed on the PDMS surface with a distance of 100 μm between them by the ink-jet microchip, the repeatabilities of the fluorescence intensity were 2.9% RSD (n = 7). The used ink-jet microchip had 4 different injection ports, and the distance between the ports was 0.995 mm. It was necessary to correct the distance in order to mix or dilute samples in a small droplet. The correction was successfully performed by moving the X-Y stage using inhouse-made software. A linear relationship was obtained between the Resorufin concentrations and the fluorescence intensity. We applied this system to an enzyme-linked immunosorbent assay (ELISA) for immunoglobulin A (IgA), and observed a difference in the fluorescence intensity derived from the amount of IgA (blank, 6.25 ng/mL, 12.5 ng/mL). These results show the usefulness of the open-type micro-analytical systems proposed by us.

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Authors and Affiliations

  1. Faculty of Urban Environmental Sciences, Tokyo Metropolitan University, 1-1 Minamiohsawa, Hachioji, Tokyo, 192-0397, Japan

    Yuki Kudo, Takako Nakahara, Tatsuro Nakagama & Katsumi Uchiyama

  2. Fine Technology Components Department, Tokyo Factory, Fuji Electric Systems Co., Ltd., 1 Fuji-machi, Hino, Tokyo, 191-8502, Japan

    Nobuko Seino & Masaki Shinoda

Authors
  1. Yuki Kudo
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  2. Takako Nakahara
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  3. Tatsuro Nakagama
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  4. Nobuko Seino
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  5. Masaki Shinoda
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  6. Katsumi Uchiyama
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Correspondence to Katsumi Uchiyama.

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Kudo, Y., Nakahara, T., Nakagama, T. et al. Development of a Surface-Reaction System in a Nanoliter Droplet Made by an Ink-jet Microchip. ANAL. SCI. 23, 91–95 (2007). https://doi.org/10.2116/analsci.23.91

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  • DOI: https://doi.org/10.2116/analsci.23.91

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