Skip to main content
SpringerLink
Log in

On-capillary Chemiluminescence Detection for Capillary Electrophoresis with a Single Capillary

  • Notes
  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

On-capillary chemiluminescence detection for capillary electrophoresis with a single capillary was reported. A hole (about 30 μm diameter) was made on the capillary wall at about 50.5 cm from the inlet end. Hydrogen peroxide solution could enter the capillary from the hole, and mixed with luminol and copper(II) to produce chemiluminescence. The chemiluminescence was detected by a PMT under the hole. Several factors that influenced chemiluminescence intensity were investigated. The detection limits for luminol and N-(4-aminolbutyl)-N-ethylisoluminol (ABEI) were 1 × 10–11 and 2 × 10–10 mol L–1, respectively. The method features simple construction and no dead volume.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. A. Amini, Electrophoresis, 2001, 22, 3107.

    Article  CAS  Google Scholar 

  2. D. T. Eash and R. J. Bushway, J. Chromatogr., A, 2000, 880, 281.

    Article  CAS  Google Scholar 

  3. D. H. Craston and M. Saeed, J. Chromatogr., A, 1998, 827, 1.

    Article  CAS  Google Scholar 

  4. I. Bjoernsdottir, J. Tjoernelund, and S. H. Hansen, Electrophoresis, 1998, 19, 2179.

    Article  CAS  Google Scholar 

  5. S. Hapuarachchi, S. P. Premeau, and C. A. Aspinwall, Anal. Chem., 2006, 78, 3674.

    Article  CAS  Google Scholar 

  6. Y. H. Rezenom, J. M. Lancaster, J. L. Pittman, and S. D. Dilman, Anal. Chem., 2002, 74, 1572.

    Article  CAS  Google Scholar 

  7. W. R. Jin, Q. Dong, D. Q. Yu, X. Y. Ye, and W. Li, Electrophoresis, 2000, 21, 1540.

    Article  CAS  Google Scholar 

  8. P. D. Voegel, W. H. Zhou, and R. P. Baldwin, Anal. Chem., 1997, 69, 951.

    Article  CAS  Google Scholar 

  9. M. Hashimoto, K. Tsukagoshi, R. Nakajima, K. Kondo, and A. Arai, J. Chromatogr., A, 2000, 867, 271.

    Article  CAS  Google Scholar 

  10. B. F. Liu, M. Ozaki, Y. Utsumi, T. Hattori, and S. Terabe, Anal. Chem., 2003, 75, 36.

    Article  CAS  Google Scholar 

  11. X.-Z. Wu, M. Suzuki, T. Sawada, and T. Kitamori, Anal. Sci., 2000, 16, 321.

    Article  CAS  Google Scholar 

  12. K. Tsukagoshi, K. Nakahama, and R. Nakajima, Anal. Chem., 2004, 76, 4410.

    Article  CAS  Google Scholar 

  13. K. Tsukagoshi, T. Nakamura, and R. Nakajima, Anal. Chem., 2002, 74, 4109.

    Article  CAS  Google Scholar 

  14. M. Hashimoto, K. Tsukagoshi, R. Nakajima, and K. Kondo, J. Chromatogr., A, 1999, 852, 597.

    Article  CAS  Google Scholar 

  15. R. Dadoo, A. G. Seto, L. A. Colón, and R. N. Zare, Anal. Chem., 1994, 66, 303.

    Article  CAS  Google Scholar 

  16. M. Hashimoto, K. Tsukagoshi, R. Nakajima, and K. Kondo, J. Chromatogr., A, 1999, 832, 191.

    Article  CAS  Google Scholar 

  17. K. Tsukagoshi, K. Sawanoi, and R. Nakajima, Talanta, 2006, 68, 1071.

    Article  CAS  Google Scholar 

  18. S. Y. Liao and C. W. Whang, J. Chromatogr., A, 1996, 736, 247.

    Article  CAS  Google Scholar 

  19. K. Tsukagoshi, Y. Ouji, and R. Nakajima, Anal. Sci., 2001, 17, 1003.

    Article  CAS  Google Scholar 

  20. M. Hashimoto, T. Nakamura, K. Tsukagoshi, R. Nakajima, and K. Kondo, Bull. Chem. Soc. Jpn., 1999, 72, 2673.

    Article  CAS  Google Scholar 

  21. K. Tsukagoshi, S. Fujimura, and R. Nakajima, Anal. Sci., 1997, 13, 279.

    Article  CAS  Google Scholar 

  22. K. Tsukagoshi, Y. Okumura, and R. Nakajima, J. Chromatogr., A, 1998, 813, 402.

    Article  CAS  Google Scholar 

  23. M. A. Ruberto and M. L. Grayeski, Anal. Chem., 1992, 64, 2758.

    Article  CAS  Google Scholar 

  24. Z. L. Gong, Y. Zhang, H. Zhang, and J. K. Cheng, J. Chromatogr., A, 1999, 855, 329.

    Article  CAS  Google Scholar 

  25. K. Tsukagoshi, K. Miyamoto, E. Saiko, R. Nakajima, T. Hara, and K. Fujinaga, Anal. Sci., 1997, 13, 639.

    Article  CAS  Google Scholar 

  26. G. A. Forbes, T. A. Nieman, and J. V. Sweedler, Anal. Chim. Acta, 1997, 347, 289.

    Article  CAS  Google Scholar 

  27. N. W. Barnett, B. J. Hindson, and S. W. Lewis, Analyst, 2000, 125, 91.

    Article  CAS  Google Scholar 

  28. J. M. Lin, H. Goto, and M. Yamada, J. Chromatogr., A, 1999, 844, 341.

    Article  CAS  Google Scholar 

  29. K. Robards and P. J. Worsfold, Anal. Chim. Acta, 1992, 266, 147.

    Article  CAS  Google Scholar 

  30. A. M. García Campaña, W. R. G. Baeyens, and Y. N. Zhao, Anal. Chem., 1997, 69, 83A.

    Article  Google Scholar 

  31. C. Kuyper and R. Milofsky, Trends Anal. Chem., 2001, 20, 232.

    Article  CAS  Google Scholar 

  32. K. Tsukagoshi, Bunseki Kagaku, 2003, 52, 1.

    Article  CAS  Google Scholar 

  33. X.-J. Huang and Z.-L. Fang, Anal. Chim. Acta, 2000, 414, 1.

    Article  CAS  Google Scholar 

  34. L.-H. Zhang and X.-Z. Wu, Anal. Chem., 2007, 79, 2562.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Materials Science and Engineering, School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui, 910-8507, Japan

    Cheng-Jie Zhang & Xing-Zheng Wu

Authors
  1. Cheng-Jie Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xing-Zheng Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xing-Zheng Wu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, CJ., Wu, XZ. On-capillary Chemiluminescence Detection for Capillary Electrophoresis with a Single Capillary. ANAL. SCI. 23, 743–746 (2007). https://doi.org/10.2116/analsci.23.743

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.23.743

Search

Navigation