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Identification of Herb Acanthopanax senticosus (Rupr. Et Maxim.) Harms by Capillary Electrophoresis with Electrochemical Detection

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Abstract

In this paper, a rapid, high efficient, sensitive and inexpensive approach based on a combination of simple ultrasonic extract and capillary electrophoresis (CE) separation with electrochemical detection (ED), is described to identify herbs by comparing their CE-ED profiles (namely, CE-ED electropherograms). The proposed method takes advantage of ultra-small sample volume, low consumption of organic solvent, simple sample pretreatment and easy cleanup procedure. It was applied to analyze the CE-ED profiles of stems of herb Acanthopanax senticosus (Rupr. Et Maxim.) Harms from different sources and different parts (roots, rhizomes, stems and leaves) of this herb. By comparing peak number, peak height and peak height ratio, we found that the CE-ED profiles showed big differences for the herbs from the different sources and the different parts of this herb. In addition, the distribution of bioactive compounds (isofraxidin, rutin and chlorogenic acid) in the different parts of this herb and their content variations affected by the source were studied with the CE-ED method. Based on their own unique CE-ED profiles, these herbs from the different sources and the different parts of this herb could be easily distinguished. Therefore, the proposed approach could be used as a rapid, high efficient and sensitive method for the identification of herbal medicines.

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References

  1. P. Drašar and J. Moravcova, J. Chromatogr., B, 2004, 812, 3.

    Article  Google Scholar 

  2. Y. X. Zhou, H. M. Lei, Y. H. Xu, L. X. Wei, and X. F. Xu, “Research Technology of Fingerprint of Chinese Traditional Medicine”, 2002, Press of Chemical Industry, Beijing, China.

    Google Scholar 

  3. S. K. Yan, W. F. Xin, G. A. Luo, Y. M. Wang, and Y. Y. Cheng, J. Chromatogr., A, 2005, 1090, 90.

    Article  CAS  Google Scholar 

  4. J. Meng, K. S. Y. Leung, Z. H. Jiang, X. P. Dong, and Z. Z. Zhao, Chem. Pharm. Bull., 2005, 53, 1484.

    Article  CAS  Google Scholar 

  5. Y. Q. Xu, S. Q. Sun, X. F. Feng, and S. L. Hu, Spectrosc. Spect. Anal., 2003, 23, 502.

    CAS  Google Scholar 

  6. J. Pazourek, D. Gajdošová, M. Spanilá, M. Farková, K. Novotná, and J. Havel, J. Chromatogr., A, 2005, 1081, 48.

    Article  CAS  Google Scholar 

  7. R. Wang, S. W. Liang, J. B. Wei, and X. F. Liu, Chin. J. Experimental Traditional Medical Formulae, 2004, 10, 61.

    Google Scholar 

  8. M. Gu, S. F. Zhang, Z. G. Su, Y. Chen, and F. Ouyang, J. Chromatogr., A, 2004, 1057, 133.

    Article  CAS  Google Scholar 

  9. Y. Sun, T. Guo, Y. Sui, and F. M. Li, J. Chromatogr., B, 2003, 792, 147.

    Article  CAS  Google Scholar 

  10. X. G. Zhou, C. Y. Zheng, J. Y. Sun, and T. Y. You, J. Chromatogr., A, 2006, 1109, 152.

    Article  CAS  Google Scholar 

  11. Y. L. Zhou, S. L. Dong, and S. Q. Nie, “Flora of Woods in Heilongjiang Province”, 1986, Heilongjiang Scientific and Technical Press, Harbin, China.

    Google Scholar 

  12. Heilongjiang Institute of Traditional Chinese Medicine, “Chinese Ciwujia Studies”, 1981, Heilongjiang Scientific and Technical Press, Harbin, China, 5.

    Google Scholar 

  13. The Pharmacopoeia Commission of PRC, “Chinese Pharmacopoeia”, 2005, Vol. 1, Chemical Industry Press, Beijing, China, 143.

    Google Scholar 

  14. M. Zhao, Y. Wang, and L. Kang, Chin. J. Chin, Materia Medica, 2001, 26, 534.

    CAS  Google Scholar 

  15. G. Zgórka and S. Kawka, J. Pharm. Biomed. Anal., 2001, 24, 1065.

    Article  Google Scholar 

  16. T. Fujikawa, H. Soya, H. Hibasami, H. Kawashima, H. Takeda, S. Nishibe, and K. Nakashima, Phytother. Res., 2002, 16, 474.

    Article  Google Scholar 

  17. Y. Kimura and M. Sumiyoshi, J. Ethnopharmacol., 2004, 95, 447.

    Article  Google Scholar 

  18. T. Fujikawa, S. Miguchi, N. Kanada, N. Nakai, M. Ogata, I. Suzuki, and K. Nakashima, J. Ethnopharmacol., 2005, 97, 375.

    Article  Google Scholar 

  19. M. L. Chen, F. R. Song, M. Q. Guo, Z. Q. Liu, and S. Y. Liu, Rapid Commun. Mass Spectrom., 2002, 16, 264.

    Article  CAS  Google Scholar 

  20. S. M. Zhang, H. W. Wang, and Y. Q. Liu, J. Shenyang Pharm. Univ., 1996, 13, 216.

    Google Scholar 

  21. A. Tolonen, T. Joutsamo, S. Mattlla, T. Kämäräinen, and J. Jalonen, Phytochem. Anal., 2002, 13, 316.

    Article  CAS  Google Scholar 

  22. S. Apers, T. Naessens, S. V. Miert, L. Pieters, and A. Vlietinck, Phytochem. Anal., 2005, 16, 55.

    Article  CAS  Google Scholar 

  23. J. Y. Sun, C. Y. Zheng, X. L. Xiao, L. Niu, T. Y. You, and E. K. Wang, Electroanalysis, 2005, 17, 1675.

    Article  CAS  Google Scholar 

  24. G. Chen, X. H. Ding, Z. G. Cao, and J. N. Ye, Anal. Chim. Acta, 2000, 408, 249.

    Article  CAS  Google Scholar 

  25. G. Chen, H. F. Luo, J. N. Ye, and C. Q. Hu, Tatanta, 2001, 54, 1067.

    CAS  Google Scholar 

  26. W. J. Zheng, S. F. Wang, X. G. Chen, and Z. D. Hu, Talanta, 2003, 60, 955.

    Article  CAS  Google Scholar 

  27. S. Hoffstetter-Kuhn, A. Paulus, E. Gassmann, and H. M. Widmer, Anal. Chem., 1991, 63, 1541.

    Article  CAS  Google Scholar 

  28. G. Chen, H. W. Zhang, and J. N. Ye, Anal. Chim. Acta, 2000, 423, 69.

    Article  CAS  Google Scholar 

  29. Q. C. Chu, T. Wu, L. Fu, and J. N. Ye, Microchim. Acta, 2004, 148, 311.

    Article  CAS  Google Scholar 

  30. A. F. Wang, Y. Zhou, F. Wu, P. G. He, and Y. Z. Fang, J. Pharm. Biomed. Anal., 2004, 35, 959.

    Article  Google Scholar 

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Authors and Affiliations

  1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China

    Xiaoguang Zhou, Jianshe Huang & Tianyan You

  2. Life Science College, Heilongjiang University, Harbin, 150080, P. R. China

    Chunying Zheng

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  1. Xiaoguang Zhou
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  2. Chunying Zheng
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  3. Jianshe Huang
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  4. Tianyan You
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Correspondence to Tianyan You.

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Zhou, X., Zheng, C., Huang, J. et al. Identification of Herb Acanthopanax senticosus (Rupr. Et Maxim.) Harms by Capillary Electrophoresis with Electrochemical Detection. ANAL. SCI. 23, 705–711 (2007). https://doi.org/10.2116/analsci.23.705

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  • DOI: https://doi.org/10.2116/analsci.23.705

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