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Integrated Rate Equation Considering Product Inhibition and Its Application to Kinetic Assay of Serum Ethanol

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Abstract

Kinetic assay of serum ethanol was investigated by predicting maximal product absorbance at 340 nm (Amk) through fitting to the yeast alcohol dehydrogenase reaction curve with the integrated rate equation, taking into account product inhibition in the presence of semicarbazide. Predicted Amk linearly responded to the preset constant of steady-state concentration of acetaldehyde (Cald). An exponential correlation function was established between desired Cald and putative Amk for authentic ethanol. For unknown samples, iterative fitting to reaction curve till preset constant Cald and resultant Amk satisfied this exponential correlation function yielded Amk with variation coefficient <4.3%. Variations in enzyme activity, data range and kinetic parameters showed negligible effects. The recovery was consistent to 100% with resistance to methanol and isopropanol. The upper limit of linear response for Amk was about 40 times of the lower limit. These results indicated that this kinetic method was reliable for serum ethanol assays with obvious advantages.

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Authors and Affiliations

  1. Laboratory of Bioinformatics & Molecular Engineering, Chongqing Key Laboratory of Biochemistry & Molecular Pharmacology, College of Pharmaceutical Sciences, Chongqing Medical University, Chongqing, 400016, People’s Republic of China

    Fei Liao, Li-Na Zhao, Yun-Sheng Zhao & Jia Tao

  2. Department of Biochemistry & Molecular Biology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016, People’s Republic of China

    Yuping Zuo

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  1. Fei Liao
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  2. Li-Na Zhao
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  3. Yun-Sheng Zhao
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  4. Jia Tao
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  5. Yuping Zuo
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Correspondence to Fei Liao.

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Liao, F., Zhao, LN., Zhao, YS. et al. Integrated Rate Equation Considering Product Inhibition and Its Application to Kinetic Assay of Serum Ethanol. ANAL. SCI. 23, 439–444 (2007). https://doi.org/10.2116/analsci.23.439

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  • DOI: https://doi.org/10.2116/analsci.23.439

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