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DMPO-OH Radical Formation from 5,5-Dimethyl-1-pyrroline N-Oxide (DMPO) in Hot Water

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Abstract

When an aqueous solution of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) was heated at 70˚C for 30 min, formation of DMPO-OH was observed by ESR. This DMPO-OH radical formation was suppressed under an argon atmosphere. When water was replaced with ultra-pure water for ICP-MS experiments, DMPO-OH radical formation was also diminished. Under an argon atmosphere in ultra-pure water, the intensity of the DMPO-OH signal decreased to about 1/20 of that observed under aerobic conditions with regular purified water. The addition of hydroxyl radical scavengers such as mannitol did not affect the formation of DMPO-OH, but the signal turned faint in the presence of EDTA. We suggest that DMPO reacted with dissolved oxygen to form DMPO-OH.

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References

  1. “Merck Index”, 20th ed., 2001, Merck & Co. Inc., NJ, USA, 597.

  2. M. Ogata, D. Kaneya, K. Shin-ya, L. Li, Y. Abe, H. Kato, S. Seki, Y. Seki, R. Gonda, S. Urano, and T. Endo, Chem. Pharm. Bull., 2005, 93, 1167.

    Article  Google Scholar 

  3. K. Makino, A. Hagi, H. Ide, and A. Murakami, Can. J. Chem., 1992, 70, 2818.

    Article  CAS  Google Scholar 

  4. J. R. Harbour, V. Chow, and J. R. Bolton, Can. J. Chem., 1974, 52, 3549.

    Article  CAS  Google Scholar 

  5. P. R. Marrriot, M. J. Parkins, and D. Griller, Can. J. Chem., 1980, 58, 803.

    Article  Google Scholar 

  6. G. R. Buettner and L. M. Oberley, Biochem. Biophys. Res. Commun., 1978, 83, 69.

    Article  CAS  Google Scholar 

  7. B. Tuccio, R. Lauricella, J. C. Bouteiller, and P. Tordo, J. Chem. Soc. Perkin Trans., 1995, 2, 295.

    Article  Google Scholar 

  8. F. A. Villamena, J. K. Merle, C. M. Hadad, and J. L. Zweier, J. Phys. Chem. A, 2005, 109, 6089.

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Kyoritsu University of Pharmacy, 1-5-30 Shibakoen, Minato, Tokyo, 105-8512, Japan

    Tomoko Shoji, Linxiang Li, Yoshihiro Abe, Yoshihisa Ishimoto, Ryoko Gonda, Tadahiko Mashino & Masataka Mochizuki

  2. Faculty of Pharmaceutical Sciences, Aomori University, 2-3-1 Koubata, Aomori, 030-0943, Japan

    Masahiro Ogata

  3. Tokyo Metropolitan Industrial Technology Research Institute, 3-13-10 Nishigaoka, Itabashi, Tokyo, 115-8586, Japan

    Michihisa Uemoto

  4. Faculty of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho, Nagoya, 467-8603, Japan

    Naoki Miyata

Authors
  1. Tomoko Shoji
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  2. Linxiang Li
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  3. Yoshihiro Abe
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  4. Masahiro Ogata
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  5. Yoshihisa Ishimoto
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  6. Ryoko Gonda
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  7. Tadahiko Mashino
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  8. Masataka Mochizuki
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  9. Michihisa Uemoto
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  10. Naoki Miyata
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Shoji, T., Li, L., Abe, Y. et al. DMPO-OH Radical Formation from 5,5-Dimethyl-1-pyrroline N-Oxide (DMPO) in Hot Water. ANAL. SCI. 23, 219–221 (2007). https://doi.org/10.2116/analsci.23.219

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  • DOI: https://doi.org/10.2116/analsci.23.219

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