Abstract
Messenger RNA (mRNA) poly(A)+RNA (from mouse kidney) was immobilized on a N-hydroxysuccinimide(NHS)-activated carboxylic acid modified electrode prepared by the treatment of a gold electrode with 3,3′-dithiodipropionic acid, followed by NHS and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). An electrochemical measurement using this mRNA electrode was carried out in an electrolyte containing ferrocenylnaphthalene diimide (1), and showed an electrochemical signal based on 1 concentrated on immobilized mRNA. After treating this electrode with water containing varied amounts of ribonuclease A (RNase A), the current peak based on 1 decreased with increasing in the amount of RNase A with a linear correlation in the range of 0.2 - 10 pg of RNase A.
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Detection limit of 3.5 × 10-7 units (ca. 0.5 pg) of RNase A is reported for RNaseAlert® Kit on the internet website of Applied Biosystems http://www.ambion.com/catalog/CatNum.php?AM1964.
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Kanazawa, M., Sato, S., Ohtsuka, K. et al. Ferrocenylnaphthalene Diimide-based Electrochemical Ribonuclease Assay. ANAL. SCI. 23, 1415–1419 (2007). https://doi.org/10.2116/analsci.23.1415
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DOI: https://doi.org/10.2116/analsci.23.1415