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MPAI (Mass Probes Aided Ionization) Method for Total Analysis of Biomolecules by Mass Spectrometry

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Abstract

We have designed and synthesized various mass probes, which enable us to effectively ionize various molecules to be detected with mass spectrometry. We call the ionization method using mass probes the “MPAI (mass probes aided ionization)” method. We aim at the sensitive detection of various biological molecules, and also the detection of bio-molecules by a single mass spectrometry serially without changing the mechanical settings. Here, we review mass probes for small molecules with various functional groups and mass probes for proteins. Further, we introduce newly developed mass probes for proteins for highly sensitive detection.

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References

  1. J. B. Fenn, M. Mann, C. K. Meng, S. F. Wong, and C. M. Whitehouse, Science, 1989, 246, 64.

    Article  CAS  Google Scholar 

  2. R. D. Smith, J. A. Loo, C. G. Edmonds, C. J. Barinaga, and H. R. Udseth, Anal. Chem., 1990, 62, 882.

    Article  CAS  Google Scholar 

  3. M. Karas, D. Bachmann, and F. Hillenkamp, Anal. Chem., 1985, 57, 2935.

    Article  CAS  Google Scholar 

  4. K. Tanaka, H. Waki, Y. Ido, S. Akita, Y. Yoshida, T. Yoshida, and T. Matsuo, Rapid Commun. Mass Spectrom., 1988, 2, 151.

    Article  CAS  Google Scholar 

  5. S. Barnes, L. Coward, M. Kirk, and J. Sfakianos, Proceedings of the Society for Experimental Biology and Medicine, 1998, 217, 254.

    Article  CAS  Google Scholar 

  6. J. F. Stevens, A. W. Taylor, and M. L. Deinzer, J. Chromatogr., A, 1999, 832, 97.

    Article  CAS  Google Scholar 

  7. T. Okada, T. Takechi, H. Wakiguchi, T. Kurashige, K. Sugahara, and H. Kodama, Arch. Biochem. Biophys., 1993, 305, 385.

    Article  CAS  Google Scholar 

  8. K. Joutsiniemi, P. Leppala, and P. Vainiotalo, Rapid Commun. Mass Spectrom., 1998, 12, 231.

    Article  CAS  Google Scholar 

  9. M. G. Ikonomou, A. T. Blades, and P. Kebarle, Anal. Chem., 1991, 63, 1989.

    Article  CAS  Google Scholar 

  10. D. S. Ashton, C. R. Beddell, D. J. Cooper, B. N. Green, and R. W. A. Oliver, Org. Mass Spectrom., 1993, 28, 721.

    Article  CAS  Google Scholar 

  11. Z. L. Cheng, K. W. M. Siu, R. Guevremont, and S. S. Berman, J. Am. Soc. Mass Spectrom., 1992, 3, 281.

    Article  CAS  Google Scholar 

  12. G. J. van Berkel, S. A. McLuckey, and G. L. Glish, Anal. Chem., 1991, 63, 2064.

    Article  Google Scholar 

  13. G. J. van Berkel, S. A. McLuckey, and G. L. Glish, Anal. Chem., 1992, 64, 1586.

    Article  Google Scholar 

  14. A. Honda, Y. Suzuki, and K. Suzuki, Chem. Rec., 2006, 6, 100.

    Article  CAS  Google Scholar 

  15. Y. Suzuki and K. Suzuki, Kagaku to Kogyo, 2003, 54, 433.

    CAS  Google Scholar 

  16. Y. Suzuki, N. Tanji, C. Ikeda, A. Honda, K. Ookubo, D. Citterio, and K. Suzuki, Anal. Sci., 2004, 20, 475.

    Article  CAS  Google Scholar 

  17. H. Kasai and S. Nishimura, Nucleic Acids Res., 1984, 12, 2137.

    Article  CAS  Google Scholar 

  18. A. Honda, H. Hifumi, Y. Honma, N. Tanji, Y. Suzuki, and K. Suzuki, Chem. Lett., 2006, 35, 290.

    Article  CAS  Google Scholar 

Download references

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Author notes

  1. Yoshio Suzuki

    Present address: Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology, c/o Katayanagi Advanced Research Loboratories, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo, 192-0982, Japan

Authors and Affiliations

  1. Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku, Yokohama, Kanagawa, 223-8522, Japan

    Aki Honda, Shinichiro Hayashi, Hiroki Hifumi, Yuya Honma, Noriyuki Tanji, Naoko Iwasawa, Yoshio Suzuki & Koji Suzuki

  2. CREST of Japan Science and Technology Agency, Kawaguchi Center Building, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan

    Aki Honda & Koji Suzuki

Authors
  1. Aki Honda
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  2. Shinichiro Hayashi
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  3. Hiroki Hifumi
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  4. Yuya Honma
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  5. Noriyuki Tanji
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  6. Naoko Iwasawa
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  7. Yoshio Suzuki
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  8. Koji Suzuki
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Honda, A., Hayashi, S., Hifumi, H. et al. MPAI (Mass Probes Aided Ionization) Method for Total Analysis of Biomolecules by Mass Spectrometry. ANAL. SCI. 23, 11–15 (2007). https://doi.org/10.2116/analsci.23.11

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  • DOI: https://doi.org/10.2116/analsci.23.11

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