Skip to main content
SpringerLink
Log in

Large-Volume Sample Sweeping with a High Theoretical Plate Number Using a Coupled-Capillary in Capillary Electrophoresis

  • Original Papers
  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

A large-volume sample injection (> 5 μL) with an extremely high theoretical plate number (N > 107) was achieved when the sweeping-MEKC mode and a coupled-capillary (100–50 μm i.d.) were simultaneously used in a capillary electrophoresis (CE) separation. A low-cost and compact violet-LED (~2 mW) was used as the fluorescence excitation source. As a result, the theoretical plate numbers of the detected peaks (two model compounds: naphthalene-2,3-dicarboxaldehyde derivatized-dopamine and -norepinephrine) were 1.0 × 107 and 7.4 × 106, respectively. The limits of detection (at S/N = 3) of these were determined to be 2.8 × 10−10 M (92 ppt) and 2.3 × 10−10 M (83 ppt), respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. R.-L. Chien and D. S. Burgi, Anal. Chem., 1992, 64, 1046.

    Article  CAS  Google Scholar 

  2. P. R. Broen and S. E. Geldart, J. Microcolumn Sep., 1998, 10, 65.

    Article  Google Scholar 

  3. T. Tábi, K. Magyar, and E. Szökó, Electrophoresis, 2005, 26, 1940.

    Article  Google Scholar 

  4. M.-S. Chun and D. S. Chung, Anal. Chim. Acta, 2003, 491, 173.

    Article  CAS  Google Scholar 

  5. A. M. Carro-Díaz, R. A. Lorenzo-Ferreira, and R. Cela-Torrijos, J. Chromatogr., A, 1996, 730, 345.

    Article  Google Scholar 

  6. M. Mazereeuw, U. R. Tjaden, and J. van der Greef, J. Chromatogr., 1994, 677, 151.

    Article  CAS  Google Scholar 

  7. S. Chen and M. L. Lee, Anal. Chem., 1998, 70, 3777.

    Article  CAS  Google Scholar 

  8. D. Kaniansky, M. Masár, J. Bielbiková, F. Iványi, F. Eisenbeiss, B. Stanislawski, B. Grass, A. Neyer, and M. Jöhnck, Anal. Chem., 2000, 72, 3596.

    Article  CAS  Google Scholar 

  9. J. P. Quirino and S. Terabe, Science, 1998, 282, 465.

    Article  CAS  Google Scholar 

  10. C.-M. Shih and C.-H. Lin, Electrophoresis, 2005, 26, 962.

    Article  CAS  Google Scholar 

  11. C.-M. Shih and C.-H. Lin, Electrophoresis, 2005, 26, 2165.

    Article  CAS  Google Scholar 

  12. C.-M. Shih and C.-H. Lin, Electrophoresis, 2005, 26, 3495.

    Article  CAS  Google Scholar 

  13. S. L. Delinger and J. M. Davis, Anal. Chem., 1992, 64, 1947.

    Article  CAS  Google Scholar 

  14. Y. Xiong, S. Park, and S. Swerdlow, Anal. Chem., 1998, 70, 3605.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, National Taiwan Normal University, 88 Sec. 4, Tingchow Road, Taipei, Taiwan

    Yen-Sheng Chang, Chun-Min Shih, Yen-Cheng Li & Cheng-Huang Lin

Authors
  1. Yen-Sheng Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chun-Min Shih
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yen-Cheng Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Cheng-Huang Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Cheng-Huang Lin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chang, YS., Shih, CM., Li, YC. et al. Large-Volume Sample Sweeping with a High Theoretical Plate Number Using a Coupled-Capillary in Capillary Electrophoresis. ANAL. SCI. 22, 557–561 (2006). https://doi.org/10.2116/analsci.22.557

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.22.557

Search

Navigation