Skip to main content
SpringerLink
Log in

Separation of Nucleotides with an Aqueous Mobile Phase Using pH- and Temperature-Responsive Polymer Modified Packing Materials

  • Original Papers
  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

A new method for the qualitative analysis of adenosine nucleotides (AMP, ADP, and ATP) and synthetic oligonucleotides has been proposed, utilizing a pH- and temperature-responsive polymer of N-isopropylacrylamide (NIPAAm), butyl methacrylate (BMA) and N,N-dimethylaminopropylacrylamide (DMAPAAm) as the stationary phase of HPLC. In the chromatographic system using the copolymer with ionizable groups of modified packing materials, we investigated how to separate adenosine nucleotides and oligonucleotides by temperature. The properties of the surface of the copolymer-grafted stationary phase altered from hydrophilic to hydrophobic and from charged to non-charged due to changes in the temperature and in the pH, respectively. In addition, it is possible to exhibit and hide ion-exchange groups on the polymer chain surface by temperature changes. These phenomena result from changes in the charge and hydrophobicity of the pH- and temperature-responsive polymer on the stationary surface with the controlling temperature. A pH- and temperature-responsive chromatography would be greatly useful for biopolymer and nucleotide separation and purification.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. J. Cadet and M. Weinfeld, Anal. Chem., 1993, 65, 675A.

    Article  Google Scholar 

  2. R. T. Pon, G. A. Buck, and A. J. Smith, Biotechnology, 1996, 21, 680.

    CAS  Google Scholar 

  3. P. J. Oefiner and C. G. Huber, J. Chromatogr., B, 2002, 782, 27.

    Article  Google Scholar 

  4. G. A. Buck, J. W. Fox, M. Gunthorpe, K. M. Hager, C. W. Naeve, R. T. Pon, P. S. Adams, and J. Rush, Biotechniques, 1999, 27, 528.

    Article  CAS  Google Scholar 

  5. M. Gilar and E. S. P. Bouvier, J. Chromatogr., A, 2000, 890, 167.

    Article  CAS  Google Scholar 

  6. W. J. Warren and G. Vella, Mol. Biotechnol., 1995, 4, 179.

    Article  CAS  Google Scholar 

  7. T. Horn and M. S. Urdea, Nucleic Acids Res., 1988, 16, 11559.

    Article  CAS  Google Scholar 

  8. T. L. Hill and J. W. Mayhew, J. Chromatogr., 1990, 512, 415.

    Article  CAS  Google Scholar 

  9. M. Gilar, K. J. Fountain, Y. Budman, U. D. Neue, K. R. Yardley, P. D. Rainville, R. J. Russell II, and J. C. Gebler, J. Chromatogr., A, 2002, 958, 167.

    Article  CAS  Google Scholar 

  10. G. Huang and T. R. Krugh, Anal. Biochem., 1990, 190, 21.

    Article  CAS  Google Scholar 

  11. Y. Xu and P. F. Swann, Anal. Biochem., 1992, 204, 185.

    Article  CAS  Google Scholar 

  12. J. Kopecek, J. Vacik, and D. Lim, J. Polym. Sci., 1971, tA1(9), 2801.

    Article  Google Scholar 

  13. T. Tanaka, I. Nishio, S.-T. Sun, and S. Ueno-Nishio, Science, 1981, 218, 467.

    Article  Google Scholar 

  14. Y. H. Bae, T. Okano, and S. W. Kim, J. Polym. Sci., Part B: Polym. Phys., 1990, 28, 923.

    Article  CAS  Google Scholar 

  15. T. Okano, N. Yamada, H. Sakai, and Y. Sakurai, J. Biomed. Mater. Res., 1993, 27, 1243.

    Article  CAS  Google Scholar 

  16. T. Shimizu, M. Yamato, A. Kikuchi, and T. Okano, Tissue Eng., 2001, 7, 141.

    Article  CAS  Google Scholar 

  17. M. Matsukata, T. Aoki, K. Sanui, N. Ogata, A. Kikuchi, Y. Sakurai, and T. Okano, Bioconjugate Chem., 1996, 7, 96.

    Article  CAS  Google Scholar 

  18. M. Heskins, J. E. Guillet, and E. James, J. Macromol. Sci. Chem., 1968, A2, 1441.

    Article  Google Scholar 

  19. H. Kanazawa, K. Yamamoto, Y. Matsushima, Y. Takai, A. Kikuchi, Y. Sakurai, and T. Okano, Anal. Chem., 1996, 68, 100.

    Article  CAS  Google Scholar 

  20. H. Kanazawa, Y. Kashiwase, K. Yamamoto, Y. Matsushima, A. Kikuchi, Y. Sakurai, and T. Okano, Anal. Chem., 1997, 69, 823.

    Article  CAS  Google Scholar 

  21. H. Kanazawa, T. Sunamoto, E. Ayano, Y. Matsushima, A. Kikuchi, and T. Okano, Anal. Sci., 2002, 18, 45.

    Article  CAS  Google Scholar 

  22. H. Kanazawa, Y. Kashiwase, K. Yamamoto, Y. Matsushima, N. Takai, A. Kikuchi, Y. Sakurai, and T. Okano, J. Pharm. Biomed. Anal., 1997, 15, 1545.

    Article  CAS  Google Scholar 

  23. H. Kanazawa, Y. Matsushima, and T. Okano, Trends Anal. Chem., 1998, 17, 435.

    Article  CAS  Google Scholar 

  24. K. Yamamoto, H. Kanazawa, Y. Matsushima, K. Oikawa, A. Kikuchi, Y. Sakurai, and T. Okano, Environ. Sci., 2000, 7, 47.

    CAS  Google Scholar 

  25. H. Kanazawa, T. Sunamoto, Y. Matsushima, A. Kikuchi, and T. Okano, Anal. Chem., 2000, 72, 5961.

    Article  CAS  Google Scholar 

  26. C. Sakamoto, Y. Okada, H. Kanazawa, E. Ayano, T. Nishimura, M. Ando, A. Kikuchi, and T. Okano, J. Chromatogr., A, 2004, 1030, 247.

    Article  CAS  Google Scholar 

  27. E. Ayano, H. Kanazawa, A. Kikuchi, and T. Okano, Anal. Sci., 2001, 17, i873.

    Google Scholar 

  28. K. Yamamoto, H. Kanazawa, Y. Matsushima, N. Takai, A. Kikuchi, and T. Okano, Chromatogr., 2000, 209, 21.

    Google Scholar 

  29. J. Kobayashi, A. Kikuchi, K. Sakai, and T. Okano, Anal. Chem., 2001, 73, 2027.

    Article  CAS  Google Scholar 

  30. J. Kobayashi, A. Kikuchi, K. Sakai, and T. Okano, J. Chromatogr., A, 2002, 958, 109.

    Article  CAS  Google Scholar 

  31. V. Bulmus, Z. Ding, C. J. Long, P. S. Stayton, and A. S. Hoffman, Bioconjugate Chem., 2000, 11, 78.

    Article  CAS  Google Scholar 

  32. H. Feil, Y. H. Bae, J. Feijen, and S. W. Kim, Macromolecules, 1992, 25, 5528.

    Article  CAS  Google Scholar 

  33. J. Kobayashi, Ph. D. Thesis, 2002.

  34. C. G. Huber, P. J. Oefner, and G. K. Bonn, Anal. Biochem., 1993, 212, 351.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physical Pharmaceutical Chemistry, Kyoritsu University of Pharmacy, 1-5-30 Shibakoen, Minato, Tokyo, 105-8512, Japan

    Eri Ayano, Chikako Sakamoto & Hideko Kanazawa

  2. Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawadacho, Shinjyuku, Tokyo, 162-8666, Japan

    Akihiko Kikuchi & Teruo Okano

Authors
  1. Eri Ayano
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chikako Sakamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hideko Kanazawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Akihiko Kikuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Teruo Okano
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hideko Kanazawa.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ayano, E., Sakamoto, C., Kanazawa, H. et al. Separation of Nucleotides with an Aqueous Mobile Phase Using pH- and Temperature-Responsive Polymer Modified Packing Materials. ANAL. SCI. 22, 539–543 (2006). https://doi.org/10.2116/analsci.22.539

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.22.539

Search

Navigation