Abstract
The aim of this research was to develop a simple procedure for a highly sensitive determination of low-molecular-weight (LMW) carbonyl compounds in drinking water and natural water. We employed a preconcentration HPLC system with 2,4-dinitrophenylhydrazine (DNPH) for the determination of LMW carbonyl compounds. A C-18 reverse-phase preconcentration column was used instead of a sample loop at the sample injection valve. A 0.1 - 5.0 mL portion of the derivatized sample solution was injected with a gas-tight syringe, and a 15% acetonitrile aqueous solution was pushed through the preconcentration column to remove the unreacted excess DNPH, which caused serious interference in the determination of formaldehyde. The detection limits were 1-3 nM with a relative standard deviation of 2 - 5% for 20 nM standard solutions (n = 5). The calibration curves were essentially unaffected by coexisting sea salts. Applications to commercial mineral water, tap water, river water, pond water and seawater are presented.
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Takeda, K., Katoh, S., Nakatani, N. et al. Rapid and Highly Sensitive Determination of Low-Molecular-Weight Carbonyl Compounds in Drinking Water and Natural Water by Preconcentration HPLC with 2,4-Dinitrophenylhydrazine. ANAL. SCI. 22, 1509–1514 (2006). https://doi.org/10.2116/analsci.22.1509
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DOI: https://doi.org/10.2116/analsci.22.1509