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Effects of hydrophilic/hydrophobic blocks ratio of PEG-b-PLGA on emission intensity and stability of over-1000 nm near-infrared (NIR-II) fluorescence dye-loaded polymeric micellar nanoparticles

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Abstract

Polymeric micellar nanoparticles (PNPs) composed of an amphiphilic block copolymer formed from hydrophilic and hydrophobic blocks and over-thousand-nanometer (OTN) near-infrared (NIR) fluorescent dye are promising fluorophores for the dynamic imaging of deep tissue. In this study, we examined the effect of the ratio of hydrophilic/hydrophobic blocks of a block copolymer, poly(ethylene glycol) (PEG)-b-poly(lactide-co-glycolide) (PLGA), on the properties of OTN-PNPs encapsulating IR-1061. OTN-PNPs with a higher molecular weight of PLGA cores showed higher emission and stabilities under physiological conditions. The PEG ratio to PLGA in the block copolymer decreased the stability of OTN-PNPs probably due to the invasion of water molecules into the polymer core. The results show that the in vivo stability and fluorescence properties can be tuned by adjusting the chain lengths of block copolymers and estimated using in vitro assays, which evaluates the brightness retention rate of the OTN-PNPs under physiological conditions.

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Acknowledgements

This work was supported by a MEXT Grant-in-Aid for Scientific Research (A), No. 19H01179, a MEXT Grant-in-Aid for Scientific Research of Innovative Areas (Resonance Bio), No. 15H05950, of Japan, and the Center of Innovation Program “COINS” from Japan Science and Technology Agency, JST. The funders had no role in the study design, data analyses, or interpretation.

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Authors and Affiliations

  1. Tsukuba Research Laboratories, JSR Corporation, 25 Miyukigaoka, Tsukuba, Ibaraki, 305-0841, Japan

    Yuichi Ueya, Yuka Kobayashi, Takashi Matsuda & Eiji Takamoto

  2. Department of Materials Science and Technology, Faculty of Advanced Engineering, Tokyo University of Science, 6-3-1 Niijuku, Katsushika, Tokyo, 125-8585, Japan

    Masakazu Umezawa, Kotoe Ichihashi, Hisanori Kobayashi, Masao Kamimura & Kohei Soga

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  1. Yuichi Ueya
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  2. Masakazu Umezawa
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  3. Yuka Kobayashi
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Correspondence to Masakazu Umezawa or Kohei Soga.

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Advanced Publication Released Online by J-STAGE October 15, 2021.

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Ueya, Y., Umezawa, M., Kobayashi, Y. et al. Effects of hydrophilic/hydrophobic blocks ratio of PEG-b-PLGA on emission intensity and stability of over-1000 nm near-infrared (NIR-II) fluorescence dye-loaded polymeric micellar nanoparticles. ANAL. SCI. 38, 199–205 (2022). https://doi.org/10.2116/analsci.21P283

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