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Crumpled Graphene/Poly (azure I) Modified Electrode for Non-enzymatic Detection of Hydrogen Peroxide Secreted from Tumor Cells

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Abstract

Hydrogen peroxide (H2O2), an important representatives of reactive oxygen species, its aberrant expression is related to many diseases, including cancers. Therefore, it is very significant to design a reliable method for the real-time detection of endogenous H2O2. Herein, we describe preparing a poly (azure I)/crumpled graphene (cGN) modified electrode by an electro-polymerization method. The results showed that this electrode presented obvious electrocatalytic effect on the reduction of H2O2. Amperometric method was employed to monitor H2O2, and the amperometric response exhibited a good linear relationship with its concentration in the range of 8.0 × 10–6–1.25 × 10–3 mol/L; the detection limit reached to 6.7 × 10–7 mol/L (S/N = 3). Furthermore, this modified also displayed good selectivity, long-term stability and a high anti-interference ability. Excitingly, the established method could be successfully used to detect H2O2 in human serum samples, and measured H2O2 secreted from living MCF-7 cells. It could have potential use in cancer diagnosis.

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Acknowledgments

We gratefully appreciate financial support from the Open Research Project of State Key Laboratory of Transducer Technology of Shanghai Institute of Microsystem and Information Technology of Chinese Academy of Sciences (SKT2003); the Key Project of Anhui Province Excellent Talent Support Program (gxyqZD2019079); PhD Research Funding of Suzhou University (2019jb27, 2019jb23); the Open Research Project of Suzhou University Research Platform (2019ykf08, 2019ykf06).

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Authors and Affiliations

  1. Anhui Key Laboratory of Spin Electron and Nanomaterials, School of Chemistry and Chemical Engineering Suzhou University, Suzhou, Anhui, 234000, China

    Keying Zhang, Ziqiang Zhang, Shangshang Ma, Deshun Cheng, Mengdi Fan, Xinyu Zhou, Fajun Li & Na Zhang

  2. State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Shanghai, 200050, China

    Keying Zhang

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  1. Keying Zhang
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  2. Ziqiang Zhang
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  3. Shangshang Ma
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  4. Deshun Cheng
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  5. Mengdi Fan
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  7. Fajun Li
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  8. Na Zhang
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Correspondence to Keying Zhang or Na Zhang.

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Zhang, K., Zhang, Z., Ma, S. et al. Crumpled Graphene/Poly (azure I) Modified Electrode for Non-enzymatic Detection of Hydrogen Peroxide Secreted from Tumor Cells. ANAL. SCI. 37, 1367–1372 (2021). https://doi.org/10.2116/analsci.21P003

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  • DOI: https://doi.org/10.2116/analsci.21P003

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