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Determination of Bisphenol-A in Water by Semi-Micro Column High-Performance Liquid Chromatography Using 2-Methoxy-4-(2-phthalimidinyl)-phenylsulfonyl Chloride as a Fluorescent Labeling Reagent

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Abstract

A highly sensitive method for the determination of bisphenol-A in water with semi-micro column high-performance liquid chromatography using 2-methoxy-4-(2-phthalimidinyl)phenylsulfonyl chloride as a fluorescent labeling reagent has been developed. The labeling reaction was carried out at 70°C for 20 min in borate buffer (pH 9.5). The derivative eluted at 11.6 min on a reversed-phase column with methanol–water (78:22, v/v) at a flow-rate of 0.2 ml/min. The fluorescence was monitored at 308 nm for excitation and 410 nm for emission. The detection limit (S/N = 3) was 10 fmol per injection. The labeling yield was about 95%.

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Authors and Affiliations

  1. Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, Hiroshima, 729-0292, Japan

    Yasuto Tsuruta, Hirofumi Inoue, Keiko Fukunaga, Sayaka Munemura & Misa Ozaki

  2. Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, Fukuyama, Hiroshima, 729-0292, Japan

    Masaya Ohta & Fumito Matsuura

Authors
  1. Yasuto Tsuruta
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  2. Hirofumi Inoue
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  3. Keiko Fukunaga
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  4. Sayaka Munemura
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  5. Misa Ozaki
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  6. Masaya Ohta
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  7. Fumito Matsuura
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Correspondence to Yasuto Tsuruta.

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Tsuruta, Y., Inoue, H., Fukunaga, K. et al. Determination of Bisphenol-A in Water by Semi-Micro Column High-Performance Liquid Chromatography Using 2-Methoxy-4-(2-phthalimidinyl)-phenylsulfonyl Chloride as a Fluorescent Labeling Reagent. ANAL. SCI. 21, 697–699 (2005). https://doi.org/10.2116/analsci.21.697

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  • DOI: https://doi.org/10.2116/analsci.21.697

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