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Simultaneous Light Emitting Diode-Induced Fluorescence and Contactless Conductivity Detection for Capillary Electrophoresis

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Abstract

A combined detection system of simultaneous contactless conductometric and fluorescent detection for capillary electrophoresis (CE) has been designed and evaluated. The two processes share a common detection cell. A blue light-emitting diode (LED) was used as the excitation source and an optical fiber was used to collect the emitting fluorescence for fluorescent detection (FD). Inorganic ions, fluorescein isothiocyanate (FITC)-labeled amino acids and small molecule peptides were separated and detected by the combined detector, and the detection limits (LODs) of sub-μM level were achieved.

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Authors and Affiliations

  1. Department of Micro-Instrument and Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China

    Feng Tan, Bingcheng Yang & Yafeng Guan

Authors
  1. Feng Tan
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  2. Bingcheng Yang
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  3. Yafeng Guan
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Correspondence to Yafeng Guan.

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Tan, F., Yang, B. & Guan, Y. Simultaneous Light Emitting Diode-Induced Fluorescence and Contactless Conductivity Detection for Capillary Electrophoresis. ANAL. SCI. 21, 583–585 (2005). https://doi.org/10.2116/analsci.21.583

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  • DOI: https://doi.org/10.2116/analsci.21.583

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