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Simultaneous Detection of Monoethanolamine, Diethanolamine, and Triethanolamine by HPLC with a Chemiluminescence Reaction and Online Derivatization to Tertiary Amine

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Abstract

In this paper we propose a new postcolumn detection method for compounds having primary, secondary, and tertiary amine moieties. The primary and secondary amine are delivatized by a reaction with epichlorohydrin having an epoxy moiety in a reaction coil to yield a tertiary amine with subsequent chemiluminescence detection using [Ru(bpy)3]3+. The liner values of the calibration curves of monoethanolamine (MEA), diethanolamine (DEA) and triethanolamine (TEA) were 0.02–1.0 nmol (r2 = 0.9986), 0.02–0.5 nmol (r2 = 0.9993) and 0.1–1.0 nmol (r2 = 0.9482), respectively. Also, the detection limits (S/N = 3) of MEA, DEA and TEA were 30, 25 and 40 pmol, respectively. The amount of DEA and TEA in shaving cream (60 μg/20 μL) were found to be 0.3 nmol and 14 nmol, respectively, by the proposed method.

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Authors and Affiliations

  1. Department of Applied Science, Okayama University of Science, 1-1 Ridaicho, Okayama, 700-0005, Japan

    Nobumitsu Niina, Kayoko Uozumi, Yuki Kokufu & Shigeo Yamazaki

  2. Division of Natural Environment and Chemistry, Faculty of Human Development, Kobe University, 3-11 Tsurukabuto, Nada, Kobe, 657-8501, Japan

    Hitoshi Kodamatani & Keiitsu Saito

Authors
  1. Nobumitsu Niina
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  2. Hitoshi Kodamatani
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  3. Kayoko Uozumi
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  4. Yuki Kokufu
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  5. Keiitsu Saito
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  6. Shigeo Yamazaki
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Corresponding author

Correspondence to Shigeo Yamazaki.

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Niina, N., Kodamatani, H., Uozumi, K. et al. Simultaneous Detection of Monoethanolamine, Diethanolamine, and Triethanolamine by HPLC with a Chemiluminescence Reaction and Online Derivatization to Tertiary Amine. ANAL. SCI. 21, 497–500 (2005). https://doi.org/10.2116/analsci.21.497

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  • DOI: https://doi.org/10.2116/analsci.21.497

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