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Ultrafast Diagnosis of the Genetic-related Disorders Using the Combined Technologies of Multiplex PCR and Multichannel Microchip Electrophoresis

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Abstract

For the diagnosis of unexplained male infertility a multiplex PCR for 6 markers, which are well-known as candidate genes for studying male infertility and located on the human Y-chromosome, has been designed. The multiplex PCR products have been separated on a 12 channel microchip electrophoresis system, which can analyze different samples simultaneously. By combining the technologies of multiplex PCR with multichannel microchip electrophoresis, the number of the DNA markers that can be screened simultaneously is increased to be 72 marker (12 × 6) in a single run while the electrophoresis analysis time is reduced to be only 180 s.

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Authors and Affiliations

  1. Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan

    Mohammad Jabasini, Feng Xu, Guichen Ping & Maged Fouad

  2. Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Hayashi-cho 2217-14, Takamatsu, 761-0395, Japan

    Ashraf A. Ewis & Mitsuru Ishikawa

  3. Department of Human Genetics Public Health, School of Medicine, The University of Tokushima, Tokushima, 770-8505, Japan

    Toshikatsu Shinka, Yutaka Nakahori & Yoshinobu Baba

Authors
  1. Mohammad Jabasini
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  2. Ashraf A. Ewis
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  3. Feng Xu
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  4. Guichen Ping
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  5. Maged Fouad
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  6. Toshikatsu Shinka
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  7. Yutaka Nakahori
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  8. Mitsuru Ishikawa
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  9. Yoshinobu Baba
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Corresponding author

Correspondence to Ashraf A. Ewis.

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Jabasini, M., Ewis, A.A., Xu, F. et al. Ultrafast Diagnosis of the Genetic-related Disorders Using the Combined Technologies of Multiplex PCR and Multichannel Microchip Electrophoresis. ANAL. SCI. 21, 1537–1539 (2005). https://doi.org/10.2116/analsci.21.1537

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  • DOI: https://doi.org/10.2116/analsci.21.1537

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