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Rapid Discrimination of Fatty Acid Composition in Fats and Oils by Electrospray Ionization Mass Spectrometry

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Abstract

Fatty acids in 42 types of saponified vegetable and animal oils were analyzed by electrospray ionization mass spectrometry (ESI-MS) for the development of their rapid discrimination. The compositions were compared with those analyzed by gas chromatography-mass spectrometry (GC-MS), a more conventional method used in the discrimination of fats and oils. Fatty acids extracted with 2-propanol were detected as deprotonated molecular ions ([M-H]-) in the ESI-MS spectra of the negative-ion mode. The composition obtained by ESI-MS corresponded to the data of the total ion chromatograms by GC-MS. The ESI-MS analysis discriminated the fats and oils within only one minute after starting the measurement. The detection limit for the analysis was approximately 10~10 g as a sample amount analyzed for one minute. This result showed that the ESI-MS analysis discriminated the fats and oils much more rapidly and sensitively than the GC-MS analysis, which requires several tens of minutes and approximately 10-9 g. Accordingly, the ESI-MS analysis was found to be suitable for a screening procedure for the discrimination of fats and oils.

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Authors and Affiliations

  1. Criminal Investigation Laboratory, Metropolitan Police Department, 2-1-1, Kasumigaseki, Chiyoda-ku, Tokyo, 100-8929, Japan

    Shoji Kurata & Kazutaka Yamaguchi

  2. Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakamachi, Koganei, Tokyo, 184-8588, Japan

    Masatoshi Nagai

Authors
  1. Shoji Kurata
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  2. Kazutaka Yamaguchi
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  3. Masatoshi Nagai
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Correspondence to Shoji Kurata.

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Kurata, S., Yamaguchi, K. & Nagai, M. Rapid Discrimination of Fatty Acid Composition in Fats and Oils by Electrospray Ionization Mass Spectrometry. ANAL. SCI. 21, 1457–1465 (2005). https://doi.org/10.2116/analsci.21.1457

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  • DOI: https://doi.org/10.2116/analsci.21.1457

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