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In-situ Neutron Reflectometry Study on Adsorption of Glucose Oxidase at Mesoporous Aluminum Oxide Film

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Abstract

In the present study, the adsorption of glucose oxidase (GOD) to a mesoporous aluminum oxide (MAO) film was examined with in-situ neutron reflectometry (NR) measurements. The MAO film was deposited on a cover glass slip and a Si disc, and its pore structure was characterized by X-ray reflectometry (XRR) and NR. The Si disc with MAO film was applied for an in-situ NR experiment, and its NR profiles before/after adsorption of GOD were continuously measured with a flow cell. The results indicated that the negatively-charged GOD molecules hardly penetrate into the narrow pore channel (pore diameter = ca. 10 nm) with opposite surface charge.

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Authors and Affiliations

  1. Institute of Quantum Beam Science, Ibaraki University, 2-1-1 Bunkyo, Mito, Ibaraki, 310-8512, Japan

    Akira Yamaguchi & Kazuya Katayama

  2. Australian Centre for Neutron Scattering, Australian Nuclear Science and Technology Organisation (ANSTO), Lucas Heights, New South Wales, 2234, Australia

    Stephen A. Holt

Authors
  1. Akira Yamaguchi
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  2. Kazuya Katayama
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  3. Stephen A. Holt
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Correspondence to Akira Yamaguchi.

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Yamaguchi, A., Katayama, K. & Holt, S.A. In-situ Neutron Reflectometry Study on Adsorption of Glucose Oxidase at Mesoporous Aluminum Oxide Film. ANAL. SCI. 36, 1331–1335 (2020). https://doi.org/10.2116/analsci.20P160

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  • DOI: https://doi.org/10.2116/analsci.20P160

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