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BODIPY-based Fluorescent Probe for the Detection of Cysteine in Living Cells

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Abstract

Cysteine (Cys), as one of the important amino acids, plays a vital role in various physiological and pathological processes. Hence, it is meaningful to develop a convenient and sensitive detection method. Herein, a novel BODIPY-based fluorescent probe (BDP-DM) was developed, which had a higher selectivity for Cys than other amino acids, including homocysteine (Hcy) and glutathione (GSH). Ultimately, we concluded that the BDP-DM probe could be used to successfully detected intracellular Cys in living HeLa cells.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (No. 21702046), project funded by the China postdoctoral Science Foundation (2018M632757), Postdoctoral research grant in Henan Province (001803025) and Innovation Scientists and Technicians Troop Construction Projects of Henan Province (No. 20IRTSTHN020).

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Authors and Affiliations

  1. Key Laboratory for Special Functional Materials of Ministry of Education, School of Materials Science and Engineering, Henan University, Kaifeng, 475004, China

    Nannan Wang, Xin Ji, Han Wang, Xianhui Wang, Yanfang Tao, Weili Zhao & Jian Zhang

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  1. Nannan Wang
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  2. Xin Ji
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  3. Han Wang
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  4. Xianhui Wang
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  5. Yanfang Tao
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  6. Weili Zhao
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  7. Jian Zhang
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Correspondence to Jian Zhang.

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Wang, N., Ji, X., Wang, H. et al. BODIPY-based Fluorescent Probe for the Detection of Cysteine in Living Cells. ANAL. SCI. 36, 1317–1321 (2020). https://doi.org/10.2116/analsci.20P134

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