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Ultrasound Assisted Cascade Extraction of Oil, Vitamin E, and Saccharides from Roselle (Hibiscus Sabdariffa L.) Seeds

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Abstract

Roselle seeds, a waste biomass of the roselle calyx processing industry, were utilized to recover valuable compounds of oil, vitamin E, and water-soluble saccharides. Firstly, ultrasound-assisted extraction (UAE) and conventional stirring extraction were conducted for saccharide extraction, and the advantage of UAE was confirmed. Secondly, oil, vitamin E, and saccharides extracted from Vietnamese roselle seeds by UAE were analyzed for the first time. Oil of tri-, di-, and mono-glycerides, fatty acids of linoleic-, oleic-, palmitic-, and stearic-acids, vitamin E of γ- and α-tocopherol, and saccharides of sucrose, raffinose, stachyose, etc. were identified, and the amounts of these components were compared with those in other country’s roselle seeds. Thirdly, cascade extraction of oil, vitamin E, and saccharides by UAE was investigated with solvents of hexane, hexane:ethyl acetate binary solvent, and water. The results indicated that the order of using solvents was very important for high and selective extraction: the best order to recover oil (almost 100%), vitamin E (95.7%), and saccharides (86.2%) was hexane, and then water.

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Acknowledgments

The current study was financially supported by the Science and Technology Research Partnership for Sustainable Development (SATREPS, Project: Multi-beneficial Measure for the Mitigation of Climate Change by the Integrated Utilization of Biomass Energy in Vietnam and Indochina countries), JST-JICA.

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Authors and Affiliations

  1. Graduate School of Engineering, Osaka Prefecture University, Sakai, 599-8531, Japan

    Bang Anh Le

  2. Graduate School of Humanity and Sustainable System Sciences, Osaka Prefecture University, Sakai, 599-8531, Japan

    Kenji Okitsu, Kiyoshi Imamura, Norimichi Takenaka & Yasuaki Maeda

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  1. Bang Anh Le
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  2. Kenji Okitsu
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  3. Kiyoshi Imamura
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  4. Norimichi Takenaka
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  5. Yasuaki Maeda
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Correspondence to Kenji Okitsu.

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Le, B.A., Okitsu, K., Imamura, K. et al. Ultrasound Assisted Cascade Extraction of Oil, Vitamin E, and Saccharides from Roselle (Hibiscus Sabdariffa L.) Seeds. ANAL. SCI. 36, 1091–1097 (2020). https://doi.org/10.2116/analsci.20P073

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  • DOI: https://doi.org/10.2116/analsci.20P073

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