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A Water-soluble Near-infrared Fluorescent Probe for Cysteine/ Homocysteine and Its Application in Live Cells and Mice

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Abstract

A near-infrared (NIR) and water-soluble probe was synthesized and studied for the detection of Cys/Hcy in aqueous solution, living cells and mice. The probe was composed of cyanine derivative as the NIR fluorescent reporting unit and pyrimidiny-thioether moiety as the Cys/Hcy responsive unit. Treatment with Cys/Hcy induced the formation of sulfur-substituted products, then intramolecular rearrangement reaction would occur to produce amino-substituted products and resulting in enhanced red fluorescence emissions. It could be applied to sense Cys/Hcy both in solution with the detection limit of 0.17 μM (or 0.32 μM) and in living cells. Cell imaging experiments proved that such a probe exhibited good cell penetration. In addition, the probe could detect Cys/Hcy in live mice with strong turn-on fluorescent response.

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Acknowledgments

This work was supported by the Key Scientific Research Program of the Higher Education Institution of Henan Province (18A150003).

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Authors and Affiliations

  1. Key Laboratory of Natural Medicine and Immuno-Engineering of Henan Province, Henan University, Kaifeng, 475004, China

    Yuhui Men, Xiaomin Zhou, Zhijie Yan, Linqiang Niu, Yang Luo, Jiamin Wang & Jianhong Wang

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  1. Yuhui Men
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  2. Xiaomin Zhou
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Correspondence to Jiamin Wang or Jianhong Wang.

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Men, Y., Zhou, X., Yan, Z. et al. A Water-soluble Near-infrared Fluorescent Probe for Cysteine/ Homocysteine and Its Application in Live Cells and Mice. ANAL. SCI. 36, 1053–1057 (2020). https://doi.org/10.2116/analsci.20P016

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