Skip to main content
SpringerLink
Log in

Screening Method for the Quality Evaluation of Cannabidiols in Water-based Products Using Liquid Chromatography Tandem Mass Spectrometry

  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

A sensitive, useful and preliminary screening method was proposed to quantitate the containable cannabinoids most commonly included in mineral water and gummi candy products, specifically cannabidiol (CBD), cannabinol (CBN), 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THCA), cannabigerol (CBG), and cannabidiolic acid (CBDA), using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) for quality evaluations. Based on the electrospray positive ion mode, the limit of detection and the limit of quantification values were 0.2 to 0.5 ng/mL and 0.8 and 2.0 ng/mL. Samples (0.5 g) were diluted by water/methanol (50/50), to which stable isotope internal standards were added; the recovery results appeared in range from 91.3 to 101.2%. This method was applied to evaluate CBD products (6 kinds) from the Japanese market. Our survey found obvious discrepancies between the labeling and the results were overserved in products. In addition, CBN, THCA, CBG, and CBDA were not detected in full-spectrum products that contained various cannabinoids that naturally occur in the cannabis plant. Thus, it is necessary to be able to verify the accurate concentration and impurity in various CBD products from the Japanese market as quickly as possible.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. E.A. Levinsohn and K.P. Hill, J. Neurol. Sci., 2020, 411, 116717.

    Article  PubMed  Google Scholar 

  2. S.M. Schmitz, H.L. Lopez, O. Marinotti, J. Diet Sappl, 2020, 25, 1.

    Google Scholar 

  3. P.S. Cogan, Cancer Expert Rev. Clin. Pharmacol., 2019, 12, 501.

    Article  CAS  Google Scholar 

  4. J. Corroon and R. Kight, Cannabis Cannabinoid Res., 2018, 3, 190.

    Article  PubMed  Google Scholar 

  5. P. Alves, C. Amaral, N. Teixeira, G. Correia-da-Silva, Pharmacol. Res., 2020, 157, 104822.

    Article  CAS  PubMed  Google Scholar 

  6. B. Duffy, L. Li, S. Lu, L. Durocher, M. Dittmar, E. Delaney-Baldwin, D. Panawennage, D. LeMaster, K. Navarette, D. Spink, Toxics, 2020, 8, 8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. E. Jang, H. Kim, S. Jang, J. Lee, S. Baeck, S. In, E. Kim, Y.U. Kim, E. Han, Forensic. Sci. Int., 2002, 306, 110064.

    Article  Google Scholar 

  8. K. Inoue, Anal. Sci., 2020, 36, 781.

    Article  CAS  PubMed  Google Scholar 

  9. C. Citti, F. Russo, S. Sgrö, A. Gallo, A. Zanotto, F. Forni, M.A. Vandelli, A. Laganà, C.M. Montone, G. Gigli, G. Cannazza, Anal. Bioanal. Chem., 2020, 412, 4009.

    Article  CAS  PubMed  Google Scholar 

  10. W.H. Abd-Elsalam, M.A. Alsherbiny, J.Y. Kung, D.W. Pate, R. Löbenberg, Talanta, 2019, 204, 846.

    Article  CAS  PubMed  Google Scholar 

  11. M. Miura, M. Nogami, M. Sakai, M. Sato, T. Yatsushiro, Anal. Sci., 2020, 36, 1243.

    Article  CAS  PubMed  Google Scholar 

  12. Y. Otsuka, T. Shimamura, M. Sakaji, H. Arita, T. Kashiwagi, H. Ukeda, Anal. Sci., 2020, 36, 977.

    Article  CAS  PubMed  Google Scholar 

  13. G. Roda, F. Faggiani, C. Bolchi, M. Pallavicini, M. Dei Cas, Anal. Sci., 2019, 35, 479.

    Article  CAS  PubMed  Google Scholar 

  14. Y. Fujii, T. Kaga, K. Nishimura, Anal. Sci., 2019, 35, 961.

    Article  CAS  PubMed  Google Scholar 

  15. Y. Horie, A. Goto, S. Tsubuku, M. Itoh, S. Ikegawa, S. Ogawa, T. Higashi, Anal. Sci., 2019, 35, 427.

    Article  CAS  PubMed  Google Scholar 

  16. I. Di Marco Pisciottano, G. Guadagnuolo, V. Soprano, M. de Crescenzo, P. Gallo, Rapid Commun. Mass Spectrom., 2018, 32, 1728.

    Article  PubMed  Google Scholar 

  17. V. Brighenti, M. Licata, T. Pedrazzi, D. Maran, D. Bertelli, F. Pellati, S. Benvenuti, J. Chromatogr. A, 2019, 1597, 179.

    Article  CAS  PubMed  Google Scholar 

  18. R. Pacifici, E. Marchei, F. Salvatore, L. Guandalini, F.P. Busardö, S. Pichini, Clin. Chem. Lab. Med., 2017, 55, 1555.

    Article  CAS  PubMed  Google Scholar 

  19. B. Patel, D. Wene, Z.T. Fan, J. Pharm. Biomed. Anal., 2017, 146, 15.

    Article  CAS  PubMed  Google Scholar 

  20. Q. Meng, B. Buchanan, J. Zuccolo, M.M. Poulin, J. Gabriele, D.C. Baranowski, PLoS One, 2018, 13, e0196396.

    Article  PubMed  PubMed Central  Google Scholar 

  21. P.M. Sobolesky, B.E. Smith, J.A. Hubbard, J. Stone, T.D. Marcotte, D.J. Grelotti, I. Grant, R.L. Fitzgerald, Clin. Chim. Acta, 2019, 491, 30.

    Article  CAS  PubMed  Google Scholar 

  22. B.J. Gurley, T.P. Murphy, W. Gul, L.A. Walker, M. ElSohly, J. Diet., 2020, 20, 1.

    Google Scholar 

  23. M. Deville, N. Dubois, R. Denooz, C. Charlier, Forensic. Sci. Int., 2020, 310, 110234.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Pharmaceutical Sciences, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu, Shiga, 525-8577, Japan

    Shiori Takashina, Yuki Igarashi, Miki Takahashi, Yukie Kondo & Koichi Inoue

Authors
  1. Shiori Takashina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yuki Igarashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Miki Takahashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yukie Kondo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Koichi Inoue
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Koichi Inoue.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Takashina, S., Igarashi, Y., Takahashi, M. et al. Screening Method for the Quality Evaluation of Cannabidiols in Water-based Products Using Liquid Chromatography Tandem Mass Spectrometry. ANAL. SCI. 36, 1427–1430 (2020). https://doi.org/10.2116/analsci.20N015

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.20N015

Keywords

Search

Navigation