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Single-base Mutation Detection Using Neutravidin-modified Polystyrene Nanoparticle Aggregation

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Abstract

A single-base mutation assay using the non-crosslinking aggregation of neutravidin-modified polystyrene nanoparticles is described. This method requires only two steps: hybridization of biotinylated probe and sample DNAs, and then mixing with neutravidin-modified nanoparticles. The aggregation was detected within 20 min in total. A combination of the DNA non-crosslinking aggregation and biotin-avidin technology has allowed sufficient performance for the detection of single nucleotide polymorphisms.

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Authors and Affiliations

  1. Bioengineering Laboratory, RIKEN (The Institute of Physical and Chemical Research), Hirosawa 2-1, Wako, Saitama, 351-0198, Japan

    Kae Sato, Munetaka Sawayanagi, Kazuo Hosokawa & Mizuo Maeda

Authors
  1. Kae Sato
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  2. Munetaka Sawayanagi
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  3. Kazuo Hosokawa
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  4. Mizuo Maeda
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Correspondence to Mizuo Maeda.

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Sato, K., Sawayanagi, M., Hosokawa, K. et al. Single-base Mutation Detection Using Neutravidin-modified Polystyrene Nanoparticle Aggregation. ANAL. SCI. 20, 893–894 (2004). https://doi.org/10.2116/analsci.20.893

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  • DOI: https://doi.org/10.2116/analsci.20.893

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