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Spectroscopic Study on the Interaction between Methylene Blue and Chondroitin 4-Sulfate and Its Analytical Application

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Abstract

The interaction of Methylene Blue (MB) with chondroitin-4-sulfate (CHS) has been investigated using spectroscopic techniques, including UV-Vis absorption, Rayleigh resonance scattering (RRS), and circular dichroism (CD). The addition of CHS caused a decrease in the absorbance of MB at 664 nm with a new absorption band appearing at 570 nm, enhanced RRS at 314 nm and 560 nm, and also resulted in an intense CD signal at 568 nm. The Scatchard model has been applied to calculating the binding constant and the number of binding sites. The calculated parameters are consistent with the experimental results. The factors affecting the interaction were investigated. Quantitative spectroscopic methods were developed for the first time. They are based on the fact that a decrease in the absorption at 664 nm and an enhancement of the RRS intensity at 314 nm are proportional to the concentration of CHS added in a certain range. Satisfactory results were obtained on the determination of synthetic samples.

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References

  1. A. L. Stone and D. F. Bradley, Biochim. Biophys. Acta., 1967, 148, 172.

    Article  CAS  Google Scholar 

  2. A. L. Stone, Biochim. Biophys. Acta., 1967, 148, 193.

    Article  CAS  Google Scholar 

  3. H. Wan, W. Ouyang, Q. C. Jiao, Q. Liu, and S. G. Zhou, ActaChim.Sinica., 2001, 59, 2181.

    CAS  Google Scholar 

  4. S. Z. Zhang, N. Li, F. L. Zhao, K. A. Li, and S. Y. Tong, Spectrochim. Acta Part A., 2002, 58, 273.

    Article  Google Scholar 

  5. E. W. Gold, Anal. Biochem., 1979, 99, 183.

    Article  CAS  Google Scholar 

  6. K. A. Homer, L. Denbow, and D. Beighton, Anal. Biochem., 1993, 214, 435.

    Article  CAS  Google Scholar 

  7. R. Malsch, J. Harenberg, L. Piazolo, G. Huhle, and D. L. Heene, J. Chromatogr. B., 1996, 685, 223.

    Article  CAS  Google Scholar 

  8. Q. C. Jiao and Q. Liu, Spectrosc. Lett., 1998, 31, 913.

    Article  Google Scholar 

  9. Q. C. Jiao and Q. Liu, Spectrosc. Lett., 1998, 31, 1353.

    Article  CAS  Google Scholar 

  10. Q. C. Jiao and Q. Liu, Anal. Lett., 1998, 31, 1311.

    Article  CAS  Google Scholar 

  11. Q. C. Jiao, Q. Liu, C. Sun, and H. He, Talanta, 1999, 48, 1095.

    Article  CAS  Google Scholar 

  12. Q. C. Jiao and Q. Liu, Spectrochim. Acta Part A., 1999, 55, 1667.

    Article  Google Scholar 

  13. S. P. Liu, H. Q. Luo, N. B. Li, Z. F. Liu, and W. X. Zheng, Anal. Chem., 2001, 73, 3907.

    Article  CAS  Google Scholar 

  14. H. Q. Luo, S. P. Liu, Z. F. Liu, Q. Liu, and N. B. Li, Anal. Chim. Acta., 2001, 449, 261.

    Article  CAS  Google Scholar 

  15. J. E. Scott, J. Dorling, and G. Quintarelli, Biochem. J., 1964, 91, 4.

    Google Scholar 

  16. M. K. Pal and M. Chaudhuri, Makromol. Chem., 1970, 133, 151.

    Article  CAS  Google Scholar 

  17. M. D. Schoenberg and R. D. Moore, Biochim. Biophys. Acta, 1964, 83, 42.

    CAS  PubMed  Google Scholar 

  18. K. Takahashi, Z. Horiuchi, and T. Yasunaga, Bull. Chem. Soc. Jpn., 1978, 51, 1278.

    Article  CAS  Google Scholar 

  19. D. Huglin, W. Seiffert, and H. W. Zimmermann, Histochemistry, 1986, 86, 71.

    Article  CAS  Google Scholar 

  20. L. Antonov, G. Gergov, V. Petrov, M. Kubista, and J. Nygren, Talanta, 1999, 49, 99.

    Article  CAS  Google Scholar 

  21. Y. J. Wei, K. A. Li, and S. Y. Tong, Talanta, 1996, 43, 1.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China

    Li Zhang, Na Li, Fenglin Zhao & Ke’an Li

Authors
  1. Li Zhang
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  2. Na Li
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  3. Fenglin Zhao
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  4. Ke’an Li
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Correspondence to Fenglin Zhao.

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Zhang, L., Li, N., Zhao, F. et al. Spectroscopic Study on the Interaction between Methylene Blue and Chondroitin 4-Sulfate and Its Analytical Application. ANAL. SCI. 20, 445–450 (2004). https://doi.org/10.2116/analsci.20.445

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  • DOI: https://doi.org/10.2116/analsci.20.445

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