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Application of the 19F NMR Technique to Observe Binding of the General Anesthetic Halothane to Human Serum Albumin

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Abstract

19F NMR techniques were employed to characterize the binding property of the widely used general anesthetic halothane with human serum albumin (HSA). It was found that 19F1H NOE and 2D 1H-19F HOESY experiments detected intermolecular NOEs between halothane 19F and HSA protons. Measurements of the diffusion coefficients for halothane were also carried out by 1H and 19F NMR, indicating the interaction of halothane with HSA. The present results indicate that these techniques are very suitable to identify a fluorine-containing ligand binding with a protein receptor in the drug-discovery process.

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Authors and Affiliations

  1. Chemical Analysis Center, Chiba University, Yayoi, Inage, Chiba, 263-8522, Japan

    Kazuaki Shikii & Hiroko Seki

  2. Analysis Research Department, Chemical Research Laboratories, Nissan Chemical Industries Ltd., Tsuboi, Funabashi, Chiba, 274-8507, Japan

    Kazuaki Shikii

  3. NM Application & Research Group, Application & Research Center, Analytical Instruments Division, JEOL Ltd., Musashino, Akishima, Tokyo, 196-8558, Japan

    Satoshi Sakurai & Hiroaki Utsumi

  4. Department of Chemistry, Faculty of Science, Tokyo Metropolitan University, Minami-Osawa, Hachioji, Tokyo, 192-0367, Japan

    Mitsuru Tashiro

Authors
  1. Kazuaki Shikii
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  2. Satoshi Sakurai
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  3. Hiroaki Utsumi
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  4. Hiroko Seki
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  5. Mitsuru Tashiro
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Correspondence to Mitsuru Tashiro.

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Shikii, K., Sakurai, S., Utsumi, H. et al. Application of the 19F NMR Technique to Observe Binding of the General Anesthetic Halothane to Human Serum Albumin. ANAL. SCI. 20, 1475–1477 (2004). https://doi.org/10.2116/analsci.20.1475

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  • DOI: https://doi.org/10.2116/analsci.20.1475

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