Abstract
Two new specific, selective, simple and inexpensive spectroscopic methods for estimating a trace amount of chromium (Cr3+) from a multi-vitamin with multi-mineral pharmaceutical formulations were developed. The proposed methods are based on the conversion of Cr3+ to Cr6+ either by oxidation with a nitric acid–perchloric acid mixture (method I) or by fusion with an excess amount of sodium carbonate (method II), followed by the complexation of Cr6+ with 1,5-diphenylcarbazide (DPC) in a mineral acidic solution of pH 1.0 ± 0.5. The pink-colored complex was estimated at 544 nm. Both methods were found to be linear in the range of 0.1–0.8 µg/ml with a limit of detection in the range of 0.0123–0.0157 µg/ml and a limit of quantitation in the range of 0.0419–0.0525 µg/ml. Method I was found to be suitable for estimating Cr3+ species in various formulations, like tablets, capsules and syrups, while method II was found to be suitable for tablets and capsules. Satisfactory recovery from spiked samples of standard Cr3+ suggests no interference of any excipients and diverse ions present in the formulations. The developed methods were compared with AAS by ANOVA, and no significant difference was observed.
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Sankalia, J.M., Mashru, R.C., Sankalia, M.G. et al. Estimation of Trace Amounts of Chromium(III) in Multi-Vitamin with Multi-Mineral Formulations. ANAL. SCI. 20, 1321–1325 (2004). https://doi.org/10.2116/analsci.20.1321
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DOI: https://doi.org/10.2116/analsci.20.1321