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Determination of Pheophorbide a and Methylpheophorbide a by Fluorescence High Performance Liquid Chromatography: Availability of Narrow Baseline Method

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Abstract

A narrow baseline method for the simultaneous determination of pheophorbide a and methylpheophorbide a in core sediment samples has been developed by high performance liquid chromatography, A linear relationship between the concentration of methylpheophorbide a and the peak height on the narrow baseline was obtained. The relative standard deviation of measurement was found to be 2.4% (n=6) for samples containing 8.1 x10-8 mol dm-3 of methylpheophorbide a.

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Authors and Affiliations

  1. Faculty of Science and Technology, Kinki University, Kowakae, Higashiosaka, 577, Japan

    Yoshiaki Yoshitake, Kikuko Kakiuchi, Kiyotoshi Morishige & Shiro Gohda

  2. College of General Education, Kinki University, Kowakae, Higashiosaka, 577, Japan

    Tsunenobu Shigematsu & Yasuharu Nishikawa

Authors
  1. Yoshiaki Yoshitake
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  2. Kikuko Kakiuchi
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  3. Kiyotoshi Morishige
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  4. Shiro Gohda
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  5. Tsunenobu Shigematsu
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  6. Yasuharu Nishikawa
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Yoshitake, Y., Kakiuchi, K., Morishige, K. et al. Determination of Pheophorbide a and Methylpheophorbide a by Fluorescence High Performance Liquid Chromatography: Availability of Narrow Baseline Method. ANAL. SCI. 2, 67–69 (1986). https://doi.org/10.2116/analsci.2.67

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  • DOI: https://doi.org/10.2116/analsci.2.67

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