Skip to main content
SpringerLink
Log in

On-Line Deproteinization of Serum Samples for High Performance Liquid Chromatographic Analysis by Using Adsorbent Gel, Butyl Toyopearl 650-M

  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

An adsorbent gel, Butyl Toyopearl 650-M, is known to adsorb proteins in a given medium with mild hydrophobic interaction. Usability of the adsorbent gel for on-line deproteinization of HPLC analysis was investigated. The samples spiked in serum were some drugs which covered a wide range of hydrophilicity and hydrophobicity. Two methods were employed; one is for hydrophilic compounds by adsorption of protein, and the other for hydrophobic compounds by direct adsorption of analytes on the gel. Both methods gave quantitative recovery of ana-lytes regardless of the protein binding ratio; the relative standard deviation was 0.8-3.3%. However, for a mixture of anticonvulsants with a wide range of hydrophobicity, simultaneous deproteinization and analysis of all components was unsuccessful. This method of deproteinization was especially suitable for the analysis of hydrophilic compounds in serum.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. H. Yoshida, I. Morita, T. Masujima and H. Imai, Chem. Pharm. Bull., 30, 2287 (1982).

    Article  CAS  Google Scholar 

  2. H. Yoshida, I. Morita, G. Tamai, T. Masujima, T. Tsuru, N. Takai and H. Imai, Chromatographic, 19, 466 (1984).

    Article  CAS  Google Scholar 

  3. I. H. Hagestam and T. C. Pinkerton, Anal. Chem., 57, 1757 (1985).

    Article  CAS  Google Scholar 

  4. I. Morita, T. Masujima, H. Yoshida and H. Imai, Chem. Pharm. Bull., 30, 3827 (1982).

    Article  Google Scholar 

  5. I. Morita, T. Masujima, H. Yoshida and H. Imai, Bunseki Kagaku, 33, E235 (1984).

    Article  CAS  Google Scholar 

  6. I. Morita, T. Masujima, H. Yoshida and H. Imai, Anal. Biochem., 151, 358 (1985).

    Article  CAS  Google Scholar 

  7. H. Imai, H. Yoshida, T. Masujima, I. Morita, K. Matsuura, A. Nakamura, K. Katayama and H. Matsuo, Anal. Lett., 16, 1109 (1983).

    Article  CAS  Google Scholar 

  8. H. Yoshida, K. Takano, I. Morita, T. Masujima and H. Imai, Jap. J. Clin. Chem.. 12, 312 (1983).

  9. G. Tamai, I. Morita, T. Masujima, H. Yoshida and H. Imai, J. Pharm. Sci., 73, 1825 (1984).

    Article  CAS  Google Scholar 

  10. G. Tamai, H. Yoshida, H. Imai, T. Takashina, K. Kotoo, T. Fuwa, Y. Tsuchioka, H. Matsuura and G. Kajiyama, Chromatographia, 20, 671 (1985).

    Article  CAS  Google Scholar 

  11. S. Hasegawa, R. Uenoyama, T. Takeda, J. Chuma, K. Suzuki, F. Kamiyama, K. Yamazaki and S. Baba, J. Uq. Chromatogr., 7, 2267 (1984).

    Google Scholar 

  12. H. Kamiyama, M. Hayashi, H. Mikami and Y. Ishida, Bunseki Kagaku. 35, 236 (1986).

    Article  Google Scholar 

  13. T. Kondo, K. Uchida, M. Achiha, K. Maeda, T. Okuda and H. Takagi, Ann. Rep. Jpn. Clin. Chem. Soc., 24, 39 (1984).

    Google Scholar 

  14. G. Tamai, H. Yoshida and H. Imai, Chromatographia, in press.

  15. G. Tamai, H. Yoshida and H. Imai, Bunseki Kagaku, 35, 335 (1986).

    Article  CAS  Google Scholar 

  16. H. Imai, G. Tamai and S. Sakura, J. Chromatogr., in press.

  17. H. Takahagi, K. Inoue and M. Horiguchi, J. Chromatogr. 352, 369 (1986).

    Article  CAS  Google Scholar 

  18. K. Nakano, S. P. Assenza and F. R. Brown, J. Chromatogr., 233, 51 (1982).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Fukuyama University, Fukuyama, 729-02, Japan

    Gen Tamai & Hideo Imai

  2. Hiroshima University School of Medicine, Kasumi, Minami, Hiroshima, 734, Japan

    Hisanobu Yoshida

Authors
  1. Gen Tamai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hisanobu Yoshida
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hideo Imai
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tamai, G., Yoshida, H. & Imai, H. On-Line Deproteinization of Serum Samples for High Performance Liquid Chromatographic Analysis by Using Adsorbent Gel, Butyl Toyopearl 650-M. ANAL. SCI. 2, 481–485 (1986). https://doi.org/10.2116/analsci.2.481

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.2.481

Keywords

Search

Navigation