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Determination of S-Suifo Group in Rabbit Plasma

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Abstract

A simple and selective method for the determination of S-sulfo group has been developed. The principle of this method is as follows: S-sulfo group is reduced by dithiothreitol to thiol and sulfite; the latter is converted to sulfur dioxide by acidification and absorbed to monoethanolamine solution by aeration. Then an adduct is prepared by the reaction of sulfite with p-aminoazobenzene and formaldehyde and determined colorimetrically by high performance liquid chromatography. Calibration curves for S-sulfonates were linear in the range of 2.5 to 100 ώM. The present method permitted the determination of S-sulfo group in normal rabbit plasma in the range of 23.3 to 47.3 ώM.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Chiba University, Yayoi-cho, Chiba, 260, Japan

    Tadayasu Togawa, Yumi Akiyama, Kyoko Kaneko, Atsuko Nakamura & Toshio Imanari

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  1. Tadayasu Togawa
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  2. Yumi Akiyama
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  3. Kyoko Kaneko
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  4. Atsuko Nakamura
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  5. Toshio Imanari
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Togawa, T., Akiyama, Y., Kaneko, K. et al. Determination of S-Suifo Group in Rabbit Plasma. ANAL. SCI. 2, 477–480 (1986). https://doi.org/10.2116/analsci.2.477

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  • DOI: https://doi.org/10.2116/analsci.2.477

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