Skip to main content
SpringerLink
Log in

Simultaneous Determination of Three Species by Flow Injection Analytical Method Using Enzyme-Immobilized Open-Tubular Reactors

  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

A simultaneous determination of three species by a flow injection analytical (FIA) method using enzyme-immobilized open-tubular reactors in parallel is described. The design of the optimal geometry of the FIA system is especially important to avoid overlap of any pairs of peaks. Ours was carried out based on the Taylor-Aris equation. Despite a somewhat complex splitting-confluence flow system, reproducible results were obtained in the simultaneous determination of L-lactate, β-D-glucose and glycerol by the detection of the resulting common product, NADH. Assignment of the enzyme reactors to the channels should be made by taking into account the concentration ranges of the analytes to be measured and the differences in the rates of individual enzyme reactions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. F. Morishita, Y. Nishikawa and T. Kojima, Bunseki Kagaku, 35, 575 (1986).

    Article  Google Scholar 

  2. E. H. Hansen, J. Ružička and A. K. Ghose, Anal. Chim. Acta, 100, 151 (1978).

    Article  CAS  Google Scholar 

  3. J. Slanina, F. Bakker, A. Bruyn-Hes and J. J. Mols, Anal. Chim. Acta, 113, 331 (1980).

    Article  CAS  Google Scholar 

  4. A. Fernandez, M. A. Luque de Castro and M. Valcárcel, Anal. Chem., 56, 1146 (1984).

    Article  CAS  Google Scholar 

  5. M. D. Luque de Castro and M. V. Cases, Analyst [London], 100, 413 (1984).

  6. M. Masoom and A. Townshend, Anal. Chim. Acta, 171, 185 (1985).

    Article  CAS  Google Scholar 

  7. T. Kojima, Y. Hara and F. Morishita, Bunseki Kagaku, 32, E101 (1983).

    Article  CAS  Google Scholar 

  8. F. Morishita, Y. Hara and T. Kojima, Bunseki Kagaku., 33, 643 (1984).

    Article  Google Scholar 

  9. R. S. Deelder, M. G. F. Kroll. A. J. B. Beeren and J. H. M. van den Berg, J. Chromatogr., 149, 669 (1978).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Yoshida, Kyoto, 606, Japan

    Fujio Morishita, Yasuhiko Nishikawa & Tsugio Kojima

Authors
  1. Fujio Morishita
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yasuhiko Nishikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tsugio Kojima
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Morishita, F., Nishikawa, Y. & Kojima, T. Simultaneous Determination of Three Species by Flow Injection Analytical Method Using Enzyme-Immobilized Open-Tubular Reactors. ANAL. SCI. 2, 411–415 (1986). https://doi.org/10.2116/analsci.2.411

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.2.411

Keywords

Search

Navigation