Abstract
Ferrocene (Fc) and β-cyclodextrin (βCyD) were modified at each end of stem-loop structured DNA as an electrochemical signal generator and its quencher, respectively, to give an electrochemical molecular beacon (eMB). A relatively high efficiency of signal quenching was achieved by an inclusion complex (βCyD ⊃ Fc) formation that was induced on the stem structure of the closed form (= stem-loop structure) of eMB. With the addition of target DNA, the structure of eMB opened to form a linear duplex, where the Fc dissociated from the βCyD to restore its intrinsic electrochemical signal. The signal contrast of the electric current for this off/on-type sensor was high, ca. 95. This technique did not require any modification of the electrode surface, and it realized the detection of the target nucleic acids in a homogeneous solution with a high sensitivity using high-performance liquid chromatography (HPLC) equipped with electrochemical detector.
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Acknowledgments
This research was partly supported by a grant-in-aid for Scientific Research (B) (No. 15H03829 to T. I.) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan. We thank Edanz Group (www.edanzediting.com/ac) for editing a draft of this manuscript.
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Kitamura, Y., Mishio, K., Arslan, P. et al. Electrochemical Molecular Beacon for Nucleic Acid Sensing in a Homogeneous Solution. ANAL. SCI. 36, 959–964 (2020). https://doi.org/10.2116/analsci.19P456
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DOI: https://doi.org/10.2116/analsci.19P456