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Preparation of a Sandwich-like Complex “MIPs-Target Molecule-Magnetic SERS Probe” and SERS Determination of Immunoglobulin G

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Abstract

Based on the reversible covalent binding between borates and glycoproteins, we proposed a scheme that can specifically recognize and detect immunoglobulin G (IgG). The application of magnetic materials to enrich SERS probes can provide an enhanced signal and repeatability. The results showed that the prepared sandwich-like complex displayed high sensitivity and excellent selectivity for IgG. The Raman peak intensity showed a good linear relationship in the concentration range of 1 mg/mL–1 ng/mL. The linear equation was y = 657.93x + 2963.9, R2 = 0.996. Parallel testing of this sandwich-like complex proved to have excellent repeatability. This method provided a new possibility for clinical non-immunity and the quantitative detection of IgG.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation Regional Science Fund Project (31560466, 81860633); Guangxi Natural Science Foundation Project (2016GXNSFAA380108).

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Authors and Affiliations

  1. Province and Ministry Co-sponsored Collaborative Innovation Center of Sugarcane and Sugar Industry, Nanning, Guangxi, 530004, China

    Xiaoxuan Li & Yuhan He

  2. Guangxi Key Laboratory- of Green Processing of Sugar Resources, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou, Guangxi, 545006, China

    Lijun Li

Authors
  1. Xiaoxuan Li
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  2. Lijun Li
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  3. Yuhan He
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Correspondence to Lijun Li.

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Li, X., Li, L. & He, Y. Preparation of a Sandwich-like Complex “MIPs-Target Molecule-Magnetic SERS Probe” and SERS Determination of Immunoglobulin G. ANAL. SCI. 36, 1025–1030 (2020). https://doi.org/10.2116/analsci.19P437

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  • DOI: https://doi.org/10.2116/analsci.19P437

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