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Comparative Study of Electrochemical Sensors Based on Enzyme Immobilized into Polyelectrolyte Microcapsules and into Chitosan Gel

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Abstract

The characteristics of an electrochemical biosensor based on a Prussian-blue screen-printed electrode containing glucose oxidase incorporated into polyelectrolyte microcapsules (PMC) are considered. PMC with the embedded enzyme were formed using sodium polystyrene sulfonate and poly(allylamine hydrochloride). The characteristics were compared with those of the enzyme immobilized in chitosan gel. We assessed the dependences of biosensor signals on the composition of the buffer solution, on the glucose concentration; the operational and long-term stabilities. The enzyme immobilized in PMC proved to be more sensitive to buffer molarity at a maximum within 35–40 mM. The apparent Michaelis constants were 1.5 and 4.1 mM at the immobilization in, respectively, chitosan and PMC. The developed biosensors were used to assay commercial juices. The biosensors' data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92–0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.

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Acknowledgments

The authors acknowledge Victor Selivanov for providing language help. The work was supported by a Russian Science Foundation grant No. 18-49-08005.

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Authors and Affiliations

  1. PSCBR RAS G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospekt Nauki 5, Pushchino, Moscow Region, 142290, Russia

    Yulia V. Plekhanova & Anatoly N. Reshetilov

  2. FSBIS Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya St. 3, Pushchino, Moscow Region, 142290, Russia

    Sergei A. Tikhonenko, Alexey V. Dubrovsky, Alexander L. Kim & Egor V. Musin

  3. Lomonosov Moscow State University, Leninskie Gory, Moscow, 119991, Russia

    Alexander L. Kim & Egor V. Musin

  4. Department of Mechanical Engineering, National Chung-Hsing University, Taichung, 402, Taiwan

    Gou-Jen Wang

  5. A. Kotelnikov Institute of Radio Engineering and Electronics, Russian Academy of Sciences, Mokhovaya St. 11-7, Moscow, 125009, Russia

    Iren E. Kuznetsova, Vladimir V. Kolesov & Anatoly N. Reshetilov

Authors
  1. Yulia V. Plekhanova
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  2. Sergei A. Tikhonenko
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  3. Alexey V. Dubrovsky
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  4. Alexander L. Kim
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  5. Egor V. Musin
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  6. Gou-Jen Wang
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  7. Iren E. Kuznetsova
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  8. Vladimir V. Kolesov
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  9. Anatoly N. Reshetilov
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Correspondence to Yulia V. Plekhanova or Anatoly N. Reshetilov.

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Plekhanova, Y.V., Tikhonenko, S.A., Dubrovsky, A.V. et al. Comparative Study of Electrochemical Sensors Based on Enzyme Immobilized into Polyelectrolyte Microcapsules and into Chitosan Gel. ANAL. SCI. 35, 1037–1043 (2019). https://doi.org/10.2116/analsci.19P131

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  • DOI: https://doi.org/10.2116/analsci.19P131

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