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Direct Determination of Benzamides in Serum by Column-Switching High-Performance Liquid Chromatography

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Abstract

A column-switching high-performance liquid chromatographic method with fluorescence detection was developed for the simultaneous determination of four benzamide-type anti-psychotic drugs: sulpiride, tiapride, sultopride and metoclopramide in human serum. In this method, a TSKgel Super-ODS column was used as an analytical column, and a TSKgel G 2000SW was prepared as a pretreatment column. Under the optimized analytical conditions, four benzamide-type anti-psychotic drugs were eluted within 18 min. The detection limits (S/N = 3) for sulpiride, tiapride, sultopride and metoclopramide are 1 ng/ml, 4 ng/ml, 2 ng/ml and 0.5 ng/ml, respectively. Finally, the method was applied to the determination of sulpiride in human serum samples obtained after a single oral dose of sulpiride.

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Authors and Affiliations

  1. Showa Pharmaceutical University, Machida, 194-8543, Japan

    Ryoko Chiba, Ayako Ogasawara & Teppei Kubo

  2. Scientific Instrument Division, Tosoh Co., 2743-1, Hayakawa, Ayase, 252-1123, Japan

    Hiroyuki Yamazaki & Masuo Umino

  3. Teikyo University School of Medicine, Mizonokuchi Hospital 3-8-3, Kawasaki, 213-0001, Japan

    Yoichi Ishizuka

Authors
  1. Ryoko Chiba
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  2. Ayako Ogasawara
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  3. Teppei Kubo
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  4. Hiroyuki Yamazaki
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  5. Masuo Umino
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  6. Yoichi Ishizuka
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Correspondence to Ryoko Chiba.

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Chiba, R., Ogasawara, A., Kubo, T. et al. Direct Determination of Benzamides in Serum by Column-Switching High-Performance Liquid Chromatography. ANAL. SCI. 19, 785–789 (2003). https://doi.org/10.2116/analsci.19.785

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  • DOI: https://doi.org/10.2116/analsci.19.785

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