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Sensing Phenothiazine Drugs at a Gold Electrode Co-modified with DNA and Gold Nanoparticles

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Abstract

DNA and gold nanoparticles are co-immobilized at a gold electrode through elaborate self-assembly processes. This configuration has proven to be useful as a sensor for phenothiazine drugs, taking advantage of the well-known, relatively large surface area of gold nanoparticles and the strong intercalation between dsDNA and phenothiazine drugs. This modified electrode has demonstrated good sensitivity and stability towards the oxidation of two model phenothiazine drugs: promethazine and chlorpromazine. A linear dependence between the concentration of phenothiazine drugs and the peak current is observed, with a concentration range of 2.0 × 10−5–1.6 × 10−4 M and 1.0 × 10−5–1.2 × 10−4 M, and a detection limit of 1.0 × 10−5 M and 7.0 × 10−6 M, for promethazine and chlorpromazine, respectively.

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References

  1. G. Erenberg, Adv. Neurol., 1992, 58, 241.

    CAS  PubMed  Google Scholar 

  2. F. Lechin, B. van der Dijs, F. Gomez, E. Acosta, and L. Arocha, Biol. Psychiatry, 1982, 17, 103.

    CAS  PubMed  Google Scholar 

  3. K. Singer and M. Wong, Postgrad. Med. J., 1970, 46, 633.

    Article  CAS  Google Scholar 

  4. M. I. Andres, G. Repetto, P. Sanz, and M. Repetto, Vet. Hum. Toxicol., 1999, 41, 273.

    CAS  PubMed  Google Scholar 

  5. C. M. Wischik, P. C. Edwards, R. Y. Lai, M. Roth, and C. R. Harrington, Proc. Natl. Acad. Sci., 1996, 93, 11213.

    Article  CAS  Google Scholar 

  6. C. Korth, B. C. May, F. E. Cohen, and S. B. Prusiner, Proc. Natl. Acad. Sci., 2001, 98, 9836.

    Article  CAS  Google Scholar 

  7. L. Amaral and J. E. Kristiansen, Int. J. Antimicrob. Agents, 2001, 18, 411.

    Article  CAS  Google Scholar 

  8. T. Mohammad and H. Morrison, J. Chromatogr. B, 1997, 704, 265.

    Article  CAS  Google Scholar 

  9. C. E. Lin and K. H. Chen, J. Chromatogr. A, 2001, 930, 155.

    Article  CAS  Google Scholar 

  10. Y. Cao, Y. J. Li, Z. Gao, and X. W. He, Acta Pharmaceutica Sinica, 1999, 34, 276.

    CAS  Google Scholar 

  11. E. Bishop and W. Hussein, Analyst, 1984, 109, 229.

    Article  CAS  Google Scholar 

  12. J. Wang, B. Freiha, and B. Deshmukh, Bioelectrochem. Bioenerg., 1985, 14, 457.

    Article  CAS  Google Scholar 

  13. J. Wang and B. Freiha, Anal. Chem., 1983, 55, 1285.

    Article  CAS  Google Scholar 

  14. M. Khadari, J. M. Kauffmann, G. Patriarche, and M. Ghandour, Electroanalysis, 1989, 1, 501.

    Article  Google Scholar 

  15. K. Stolze and R. Mason, Chemico-Biological Interactions, 1991, 77, 283.

    Article  CAS  Google Scholar 

  16. B. Fujimoto, J. Delrow, P. Health, and M. Schurr, J. Phys. Chem., 1994, 98, 6633.

    Article  CAS  Google Scholar 

  17. T. Friedmann and D. Brown, Nucleic Acids Res., 1978, 5, 6150.

    Article  Google Scholar 

  18. M. A. Hyatt, in “Colloidal Gold: Principles, Methods and Applications”, 1977, Vol. 3, Academic Press, New York.

    Google Scholar 

  19. W. S. Sutherland and J. D. Winefordner, J. Colloid. Interface Sci., 1992, 48, 129.

    Article  Google Scholar 

  20. S. Trasatti and O. A. Petrii, J. Electroanal. Chem., 1992, 327, 353.

    Article  CAS  Google Scholar 

  21. G. Tremiliosi-Filho, L. H. Dall’Antonia, and G. Jerkiewicz, J. Electroanal. Chem., 1997, 422, 149.

    Article  CAS  Google Scholar 

  22. H. M. Schessler, D. S. Karpovich, and G. J. Blanchard, J. Am. Chem. Soc., 1996, 118, 9645.

    Article  CAS  Google Scholar 

  23. D. S. Karpovich and G. J. Blanchard, Langmuir, 1994, 10, 3315.

    Article  CAS  Google Scholar 

  24. C. A. Widrig, C. J. Chung, and M. D. Porter, J. Electroanal. Chem., 1991, 310, 335.

    Article  CAS  Google Scholar 

  25. D. E. Weishaar, B. D. Lamp, and M. D. Porter, J. Am. Chem. Soc., 1992, 114, 5860.

    Article  Google Scholar 

  26. H. M. Schessler, D. S. Karpovich, and G. J. Blanchard, J. Am. Chem. Soc., 1996, 118, 9645.

    Article  CAS  Google Scholar 

  27. R. I. Baxter, G. Svehla, B. Kerr, and A. D. Woolfson, Anal. Chim. Acta, 1984, 164, 171.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry and National Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210093, P. R. China

    Jun Zhong, Han Dai, Chunhai Fan & Genxi Li

  2. Nanoarchitectonics Research Center, AIST, Central 5, 1-1-1, Higashi, Tsukuba, 305-8565, Japan

    Zhimei Qi & Naoki Matsuda

Authors
  1. Jun Zhong
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  2. Zhimei Qi
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  3. Han Dai
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  4. Chunhai Fan
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  5. Genxi Li
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  6. Naoki Matsuda
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Zhong, J., Qi, Z., Dai, H. et al. Sensing Phenothiazine Drugs at a Gold Electrode Co-modified with DNA and Gold Nanoparticles. ANAL. SCI. 19, 653–657 (2003). https://doi.org/10.2116/analsci.19.653

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  • DOI: https://doi.org/10.2116/analsci.19.653

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